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公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Cataloguenumber
ENZ-310
Description
MMLV(MoloneyMurineLeukemiaVirus)ReverseTranscriptaseisaDNApolymerasethatsynthesizesacomplementaryDNAstrandsfromsingle-strandedRNA,DNA,oranRNA-DNAhybridasatemplate.ThisrecombinantenzymewaspurifiedfromE.coli,whichcarriedamodifiedMMLV-RTgene.ComparedtoAMVReverseTranscriptase,thisenzymehasamuchweaker5"-3"ribonucleaseHactivity,whichallowsthesyntesisoflongercDNAs(>7kb).
Source
RecombinantE.colistrain.
PhysicalAppearance
SterileFilteredclearsolution(200U/µl).
Formulation
50mMTris-HCl,0.1MNaCl,0.1%TritonX-100,2mMDTT,0.1mMEDTAand50%glycerol.
StABIlity
Stablefor5daysat10°C,forlongerperiodoftimestoreat-20°C.
Pleasepreventfreeze-thawcycles.
Pleasepreventfreeze-thawcycles.
UnitDefinition
Oneunitisdefinedastheamountofenzymerequiredtocatalyzetheincorporationof1nmolofdeoxyribonucleotideintoacid-insolubleformsin10minutesat37oC,usingpoly(A)-oligo(dT)12-18asthetemplate-primer.StandardCDNASynthesisConditions50mMTris-HCl(pH8.3),75mMKCl,3mMMgCl2,10mMDTT,1.0mMeachdATP,dGTP,dCTP,anddTTP,0.2mgradomhexamer,1-5mgRNA,200unitsM-MLVRT.Thereactionvolumewas20mlandtheincubationwas45minat42oC.
5XReactionBuffer
250mMTris-HCl(pH8.3),375mMKCl,15mMMgCl2,and50mMDTT.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.