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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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商品描述
Cataloguenumber
ENZ-988
Synonyms
Tyrosine3-monooxygenase,Tyrosine3-hydroxylase,TH.
Introduction
Tyrosine3-monooxygenase(Th),isarate-limitingenzymeincatecholaminesynthesis.ThutilizestetrahydrobiopterinaswellasmolecularoxygentoconverttyrosinetoDOPA.Thregulatesdopamine(DA)neurotransmissionatthebiosynthesisandreuptakesteps.Thtakesavitalpartinthephysiologyofadrenergicneurons.FurThermore,TheffectsoverexpressioninlymphocytesonthedifferentiationaswellasfunctionofThelpercells.
Description
THMouseRecombinantproducedinSf9Baculoviruscellsisasingle,glycosylatedpolypeptidechaincontaining507aminoacids(1-498a.a.)andhavingamolecularmassof57.0kDa(MolecularsizeonSDS-PAGEwillappearatapproximately50-70kDa).
THisexpressedwitha6aminoacidHistagatC-Terminusandpurifiedbyproprietarychromatographictechniques.
Source
Sf9,Baculoviruscells.
PhysicalAppearance
SterileFilteredcolorlesssolution.
Formulation
THproteinsolution(0.25mg/ml)containingPhosphateBufferedSaline(pH7.4)and10%glycerol.
StABIlity
Storeat4°Cifentirevialwillbeusedwithin2-4weeks.
Store,frozenat-20°Cforlongerperiodsoftime.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).
Avoidmultiplefreeze-thawcycles.
Purity
Greaterthan90.0%asdeterminedbySDS-PAGE.
Aminoacidsequence
ADPMPTPSASSPQPKGFRRAVSEQDTKQAEAVTSPRFIGRRQSLIEDARKEREAAAAAAAAAVASAEPGNPLEAVVFEERDGNAVLNLLFSLRGTKPSSLSRALKVFETFEAKIHHLETRPAQRPLAGSPHLEYFVRFEVPSGDLAALLSSVRRVSDDVRSAREDKVPWFPRKVSELDKCHHLVTKFDPDLDLDHPGFSDQAYRQRRKLIAEIAFQYKQGEPIPHVEYTKEEIATWKEVYATLKGLYATHACREHLEAFQLLERYCGYREDSIPQLEDVSHFLKERTGFQLRPVAGLLSARDFLASLAFRVFQCTQYIRHASSPMHSPEPDCCHELLGHVPMLADRTFAQFSQDIGLASLGASDEEIEKLSTVYWFTVEFGLCKQNGELKAYGAGLLSSYGELLHSLSEEPEVRAFDPDTAAVQPYQDQTYQPVYFVSESFSDAKDKLRNYASRIQRPFSVKFDPYTLAIDVLDSPHTIRRSLEGVQDELHTLTQALSAISHHHHHH.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.