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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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商品描述
Cataloguenumber
HCV-244
Introduction
HCVisasmall50nm,enveloped,single-stranded,positivesenseRNAvirusinthefamilyFlaviviridae.
HCVhasahighrateofreplicationwithapproximatelyonetrillionparticlesproducedeachdayinaninfectedindividual.DuetolackofproofreADIngbytheHCVRNApolymerase,theHCVhasanexceptionallyhighmutationrate,afactorthatmayhelpiteludethehost"simmuneresponse.HepatitisCvirusisclassifiedintosixgenotypes(1-6)withseveralsubtypeswithineachgenotype.ThepreponderanceanddistributionofHCVgenotypesvariesglobally.Genotypeisclinicallyimportantindeterminingpotentialresponsetointerferon-basedtherapyandtherequireddurationofsuchtherapy.Genotypes1and4arelessresponsivetointerferon-basedtreatmentthanaretheothergenotypes(2,3,5and6).
HCVhasahighrateofreplicationwithapproximatelyonetrillionparticlesproducedeachdayinaninfectedindividual.DuetolackofproofreADIngbytheHCVRNApolymerase,theHCVhasanexceptionallyhighmutationrate,afactorthatmayhelpiteludethehost"simmuneresponse.HepatitisCvirusisclassifiedintosixgenotypes(1-6)withseveralsubtypeswithineachgenotype.ThepreponderanceanddistributionofHCVgenotypesvariesglobally.Genotypeisclinicallyimportantindeterminingpotentialresponsetointerferon-basedtherapyandtherequireddurationofsuchtherapy.Genotypes1and4arelessresponsivetointerferon-basedtreatmentthanaretheothergenotypes(2,3,5and6).
Description
TheE.coliderivedrecombinantBiotinLabeledproteincontainstheHCVNS3immunodominantregions,a.a.1450-1643,22kDa.
TheBiotinlabeledproteinisfusedwithb-galactosidase(114kDa)atN-terminus.
TheBiotinlabeledproteinisfusedwithb-galactosidase(114kDa)atN-terminus.
PurificationMethod
HCVNS3Biotinlabeledproteinwaspurifiedbyproprietarychromatographictechnique.
Purity
HCVNS3Biotinlabeledproteinis>95%pureasdeterminedby10%PAGE(coomassiestaining).
Formulation
8Murea,20mMTris-HClpH8.0,10mMb-mercaptoethanol.
StABIlity
HCVNS3Biotinalthoughstableat4°Cfor1week,shouldbestoredbelow-18°C.
Pleasepreventfreezethawcycles.
Specificity
ReactivitywithhumanHCVpositiveserumundetermined.
Applications
HCVNS3BiotinlabeledantigenissuitableforELISAandWesternblots,excellentantigenfordetectionofHCVwithminimalspecificityproblems.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.


