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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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商品描述
Cataloguenumber
HCV-251
Introduction
HCVisasmall50nm,enveloped,single-stranded,positivesenseRNAvirusinthefamilyFlaviviridae.
HCVhasahighrateofreplicationwithapproximatelyonetrillionparticlesproducedeachdayinaninfectedindividual.DuetolackofproofreADIngbytheHCVRNApolymerase,theHCVhasanexceptionallyhighmutationrate,afactorthatmayhelpiteludethehost"simmuneresponse.HepatitisCvirusisclassifiedintosixgenotypes(1-6)withseveralsubtypeswithineachgenotype.ThepreponderanceanddistributionofHCVgenotypesvariesglobally.Genotypeisclinicallyimportantindeterminingpotentialresponsetointerferon-basedtherapyandtherequireddurationofsuchtherapy.Genotypes1and4arelessresponsivetointerferon-basedtreatmentthanaretheothergenotypes(2,3,5and6).
HCVhasahighrateofreplicationwithapproximatelyonetrillionparticlesproducedeachdayinaninfectedindividual.DuetolackofproofreADIngbytheHCVRNApolymerase,theHCVhasanexceptionallyhighmutationrate,afactorthatmayhelpiteludethehost"simmuneresponse.HepatitisCvirusisclassifiedintosixgenotypes(1-6)withseveralsubtypeswithineachgenotype.ThepreponderanceanddistributionofHCVgenotypesvariesglobally.Genotypeisclinicallyimportantindeterminingpotentialresponsetointerferon-basedtherapyandtherequireddurationofsuchtherapy.Genotypes1and4arelessresponsivetointerferon-basedtreatmentthanaretheothergenotypes(2,3,5and6).
Description
TheE.coliderivedrecombinantproteincontainstheHCVNS3immunodominantregions,aminoacids1359-1456.TheproteinisfusedtoaGSTtagatN-Terminus.
PurificationMethod
HCVNS3Genotype-3proteinwaspurifiedbyproprietarychromatographictechnique.
Purity
HCVNS3Genotype-3proteinis>95%pureasdeterminedby10%PAGE(coomassiestaining).
Formulation
1.5Murea,25mMTris-HClpH-8,0.2%Triton-X&50%Glycerol.
StABIlity
HCVNS3Genotype-3althoughstableat4°Cfor1week,shouldbestoredbelow-18°C.
Pleasepreventfreezethawcycles.
Specificity
ImmunoreactivewithseraofHCV-infectedindividuals.
Applications
HCVNS3Genotype-3antigenissuitableforELISAandWesternblots,excellentantigenfordetectionofHCVwithminimalspecificityproblems.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.


