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苏州工业园区生物纳米园A4#216
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4000-520-616 / 18915418616
传真号码
0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
Cataloguenumber
ANT-218
Synonyms
Regeneratingislet-derivedprotein3alpha,RegIII-alpha,Pancreatitis-associatedprotein1,REG3A,HIP,PAP,PAP1,REG3,INGAP,PAP-H,PBCGF,REG-III.
Type
PolyclonalRabbitAntibody.
Introduction
Pancreatitis-associatedprotein(PAP)isasecretoryproteinnotnormallyexpressedinhealthypancreasbuthighlyinducedduringacutepancreatitis.WhilePAPhasbeenshowntobeanti-bacterialandantiapoptoticinvitro,itsdefinitiveBIOLOGicalfunctioninvivoisnotclear.Usingantisepseoligonucleotides,inhibitionofPAPexpressionsignificantlyworsenedpancreatitisinaratmodel.Duringpancreatitis,PAPreleasedbythepancreascouldmediatelunginflammationthroughinductionofhepaticTNF-alphaexpressionandsubsequentincreaseincirculatingTNF-alpha.
PAPisactivatedinprimarylivercancers.Innormalliver,theproteinisundetectableinnormalmaturehepatocytesandfoundonlyinsomeductularcells,representingpotentialhepaticProgenitorcells.PAPcanbeconsideredhepaticcytokinethatcombinesmitogenicandanti-apoptoticfunctionsregardinghepatocytes,andconsequentlyactsasagrowthfactorinvivotoenhanceliverregeneration.Inpancreaticcancor,PAPwasoverexpressedin79%(30of38)ofpancreaticductaladenocarcinoma,19%(7of36)ofchronicpancreatitis,and29%(2of7)ofmucinouscystadenoma.PAPwasfoundinmalignantductularstructuresinpancreaticcarcinomasaswellasinbenignproliferatingductulesandacinarcellsinchronicpancreatitis.ElevationofPAPinpatientswithpancreaticcancerisnotmerelyexplainablebyconcomitantpancreatitis,butseemstobeduetoincreasedPAPproductionbythecancercellsandisalsocorrelatedtotumourloadasexpressedbytheUICCstages.
EpithelialexpressionofPAPwasinducedunderintestinalmucosalinflammationinitiatedbyexposuretocommensalbacteriaorDSSaswellasinflamedIBDcolon.IncreasedserumlevelofPAPdiagnosedileallocationinactiveCrohndiseasewithasensitivityof60%,aspecificityof94%,apositivepredictivevalueof84%andanegativepredictivevalueof81%.ElevatedserumPAP(>50ng/mL)issignificantlyassociatedwithdiseaseactivityandileallocationofCrohndisease.
PAPisactivatedinprimarylivercancers.Innormalliver,theproteinisundetectableinnormalmaturehepatocytesandfoundonlyinsomeductularcells,representingpotentialhepaticProgenitorcells.PAPcanbeconsideredhepaticcytokinethatcombinesmitogenicandanti-apoptoticfunctionsregardinghepatocytes,andconsequentlyactsasagrowthfactorinvivotoenhanceliverregeneration.Inpancreaticcancor,PAPwasoverexpressedin79%(30of38)ofpancreaticductaladenocarcinoma,19%(7of36)ofchronicpancreatitis,and29%(2of7)ofmucinouscystadenoma.PAPwasfoundinmalignantductularstructuresinpancreaticcarcinomasaswellasinbenignproliferatingductulesandacinarcellsinchronicpancreatitis.ElevationofPAPinpatientswithpancreaticcancerisnotmerelyexplainablebyconcomitantpancreatitis,butseemstobeduetoincreasedPAPproductionbythecancercellsandisalsocorrelatedtotumourloadasexpressedbytheUICCstages.
EpithelialexpressionofPAPwasinducedunderintestinalmucosalinflammationinitiatedbyexposuretocommensalbacteriaorDSSaswellasinflamedIBDcolon.IncreasedserumlevelofPAPdiagnosedileallocationinactiveCrohndiseasewithasensitivityof60%,aspecificityof94%,apositivepredictivevalueof84%andanegativepredictivevalueof81%.ElevatedserumPAP(>50ng/mL)issignificantlyassociatedwithdiseaseactivityandileallocationofCrohndisease.
Description
TheAntibodywasraisedinrabbitsbyimmunizationwiththerecombinanthumanREG3A.TheaminoacidsequenceoftherecombinanthumanREG3Ais100%homologustotheaminoacidsequenceofthehumanREG3Asequence.Theimmunizationantigenisthe18.4kDaproteincontaining149aminoacidresidues-HisTag.
SpeciesReactivity
Human.
PurificationMethod
ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinanthumanREG3A.
Titer
DefinedbyindirectELISA,itis:>1:100,000forantibodyconcentration1mg/ml,25ngofantigenarecoatedperwell,andisthendefinedatapointofmaximaldecreaseofthetitrationcurve.
PhysicalAppearance
SterileFilteredWhitelyophilized(freeze-dried)powder.
Formulation
Sterilefilteredandlyophilizedfrom1mg/mlin0.05MPhosphatebuffer,0.1MNaCl,pH-7.2.
Solubility
Add0.1mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.
StABIlity
Storelyophilizedantibodyat-20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat-80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowanychangeaftertwoweeksat4°C.
Aminoacidsequence
MRGSHHHHHHGMASHMEEPQRELPSARIRCPKGSKAYGSHCYALFLSPKSWTDADLACQKRPSGNLVSVLSGAEGSFVSSLVKSIGNSYSYVWIGLHDPTQGTEPNGEGWEWSSSDVMNYFAWERNPSTISSPGHCASLSRSTAFLRWKDYNCNVRLPYVCKFTD.
Usage
Prospec"sproductsarefurnishedforLABORATORYRESEARCHUSEONLY.Theproductmaynotbeusedasdrugs,agriculturalorpesticidalproducts,foodadditivesorhouseholdchemicals.