Type: Goat IgG

Applications: IHC; WB; E; NB

E=ELISA; FACS; FC=Flow Cytometry; FPLC=Fast Protein Liquid Chromatography; GF=Gravity Flow; HPLC=High Performance Liquid Chromatography; ICC=Immunocytochemistry; IF=Immunofluorescence; IHC=Immunohistochemistry; IP=Immunoprecipitation; NAC=Non-adherent Cell Assays; NB=Neutralization of Bioactivity; SE=Sandwich ELISA; TPE=Targeted Protein Expression; WB=Western blotting; ; AC=Adherent Cell Assays; FM=Fluorescent Micsroscopy; ; ; BSC-CM5= Biacore Sensor Chip CM5; BSM=Biosactive Small Molecule or Peptide; CDM=Cell Differentiation Media; ; ; ; ; ; Health and Fitness; ; ; DNA Extraction/Purification; ; In vivo Like Assays

Species Reactivity: H

B=Bovine; Ca=Cat; Ch=Chicken; D=Dog; EQ=Equine; GP=Guinea Pig; H=Human; M=Mouse; P=Porcine; Pr=Primate; R=Rat; Rb=Rabbit; Y=Yeast; Xe=Xenopus; Ze=Zebrafish; ; ; ; NA-Not Applicable; STP=Step-Tactin Proteins; All

Format: Affinity Purified - liquid

Immunogen: Purified, CHO cell-derived, recombinant human Fas Ligand (rhFas Ligand).

FAS Ligand,  also known as CD95L or APT1LG1 , is a member of the TNF receptor family.

The full length Fas-L protein has a cytoplasmic domain, transmembrane (Type II) domain and extracellular domain. The membrane-bound form of Fas-L is proteolytically cleaved to produce soluble Fas-L. There are at least 2 known isoforms of Fas-L produced by alternate splicing. 

Fas ligand-receptor interactions play an important role in the regulation of the immune system and the progression of cancer.

Figure 1-The biological activity of rhFas Ligand was measured by its ability to induce apoptosis of Jurkat cells (Cifone M.G. et al., 1994, J. Exp. Med. 180:1547). The ED₅₀ of this effect is typically 0.2 - 0.5 ng/mL.

Figure 2-To measure the ability of the antibody to neutralize the bioactivity of rhFas Ligand, rhFas Ligand was incubated with various concentrations of the antibody for 1 hour at 37° C. Following this preincubation period, Jurkat cells were added. The assay mixture in a total volume of 100 μL/well, containing antibody at the concentrations indicated, rhFas Ligand at 2 ng/mL and Jurkat cells at 5 x 10⁴/mL, was incubated for 2 days at 37° C in a 5% CO₂ humidified incubator. ³H-thymidine was added during the final 4 hours of the incubation. The cells were subsequently harvested onto a glass fiber filter and the ³H-thymidine incorporation into DNA was determined. The ND₅₀ of this antibody is typically 2 - 8 ng/mL. 

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