Synonyms : CDF, HILDA, D-FACTOR, Differentiation- stimulating factor, Melanoma-derived LPL inhibitor, MLPLI, Emfilermin, Leukemia inhibitory factor, LIF, DIA. Description:Mouse Recombinant Leukemia Inhibitory Factor (LIF) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 181 amino acids and having a molecular mass of 20 kDa. Source: Escherichia coli. Purity: Greater than 95.0% as determined by SDS-PAGE. Endotoxin Level : Endotoxin level is less than 0.1 ng per g (1EU/ g) as determined by the LAL method. Biological Activity : Activity of mouse LIF was determined by its ability to induce differentiation of murine M1myeloid leukemic cells. The specific activity is 100mIU/mg, where 50U is defined as the amount of murine LIF required to induce differentiation in 50% of the M1 colonies per ml of agar culture. Amino acid Sequence: GSPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAF Formulation Storage: It was lyophilized from sterile 10 mM sodium phosphate ( pH 7.4) and we recommend that the powder be reconstituted in sterile 10 mW-cm H2O. Epidermal Growth Factor (EGF) should be stored desiccated below -20 C. Upon reconstitution, EGF should be stored at 4 C For long-term storage at -20 C, it is recommended to add a carrier protein (0.1% HSA or BSA). Freeze-thaw cycles should be avoided. Usage: The product is for research only and may not be allowed for usage as drugs, agricultural or pesticidal products, food additives or household chemicals. 百恩维生物 bio-equip.com 百恩维生物科技有限公司（BioWit Technologies），是一家专业从事生物科技前沿技术研发的高新技术企业。为国内外众多药厂和科研机构提供病毒包装，蛋白表达，载体构建，干细胞培养基，转染试剂、细胞因子等产品及技术服务。公司专家团队曾在国内外知名学府和国际一流制药公司从事研发多年。 百恩维生物在深圳宝安高新奇工业园拥有世界一流的生物学实验室，包括多种先进的实验设备，以及一批经验丰富的技术人员。为客户提供最全面的病毒包装技术服务，除了常规病毒类型，还有非整合型慢病毒，9种血清型的腺相关病毒等极有特色的病毒包装服务。 百恩维为客户生产的干细胞培养基已经进入多项临床前试验阶段，产品质量控制指标达到临床使用样品要求。百恩维生物还提供数千种重组蛋白、以及转染试剂等产品。 百恩维生物建立起完善质量保证体系，所有产品均有卓越的质量保证。 百恩维生物已经拥有自主知识产权的产品2项，发明专利技术3项，并与国内外知名大学、中科院以及医科院等知名科研机构建立了良好的合作关系，在生物尖端技术方面能够持续创新。 百恩维生物将本着 专注、创新、诚信、和谐 的企业理念，为生物技术能够为人类健康造福而不懈追求！ ●病毒包装技术服务：腺病毒、慢病毒、非整合型慢病毒、腺相关病毒、杆状病毒、逆转录病毒 ●载体构建：过表达载体、ShRNA表达载体、miRNA表达载体 ●蛋白表达与纯化：原核蛋白、真核蛋白、昆虫杆状病毒蛋白 ●干细胞培养基：干细胞无血清培养基、干细胞含血清培养基 ●转染试剂：HET、Polyfectine、UES ●细胞因子：数千种细胞因子

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