Type:Protein
SpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All
CD146(433-647a.a.)belongstotheimmunoglobulinsupergenefamilywithfiveimmunoglobulinlikedomains(V-V-C2-C2-C2),atransmembraneregionanda63residuecytoplasmictail.TheproteinisamembraneglycoproteinthatfunctionsasaCa2+independentcelladhesionmoleculeinvolvedinheterophiliccelltocellinteractions.CD146hasamolecularsizeof130kDainitsreducedform
(118kDaunreduced),andNlinkedglycosylationaccountsforfiftypercentoftheapparentmolecularweight.Insomecellsthemoleculecarriesasulfate3glucuronylmoiety.
Applications:
•ELISA
•InhibitionAssays
•WesternBlotting-OnSDS-PAGEcommassiebluestainedgel,thepurifiedrecombinantproteinshowsabandat47kDa.
Image:CD146structureandsequence
References:
1.RoleofCD146indetectionofapoptoticcirculatingendothelialcellsinobstructivesleepapnea. AmJRespirCritCareMed2007Jun15;175(12):1346-1347;authorreply1347
2.ElevationofserumADIponectinandCD146levelsindiabeticnephropathy. DiabetesResClinPract2007May8.
3.RestorationofsolubleCD146inpatientswithCrohn"sdiseasetreatedwiththeTNF-alphaantagoNISTinfliximab. InflammBowelDis2007May2.
4.VisualizationofCD146dimerizationanditsregulationinlivingcells. BiochimBiophysActa2007Apr;1773(4):513-20.
5.PoorprognosisinbreastcarcinomascorrelateswithincreasedexpressionoftargetableCD146andc-Metandwithproteomicbasal-likephenotype. HumPathol2007Jun;38(6):830-41
6.Detectionofcirculatingendothelialcells:CD146-basedmagneticseparationenrichmentorflowcytometricassay? JClinOncol2007Feb10;25(5):e1-2;authorreplye3-5
