Type:BlockingBuffer
Applications:E
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysFormat:liquid
NeptuneBlockcontainsaproprietarynon-mammalianformulationbasedonfishproteins,plusmolecularstABIlizersthatprovideahighdegreeofblockingefficiency.NeptuneBlockwasdesignedforantigen-downELISAformats,andforsandwichassayswithhighbackgroundproblems.
Thisformulationisaheterogeneousmixtureofsmallmoleculescapableofblockingboththenonspecificbindingsitesoftheadsorbedprotein,aswellastheunoccupiedregionsofthepolystyreneplatethatarenotstericallyaccessIBLetolargertrADItionalmammalianblockingreagents,therebyreducingbackgroundnoise.Thesmallsizeoftheseuniqueblockersresultsinminimalsterichindrancetokeyepitoperegionsoftargetcoatedproteins,thusenhancingspecificsignalgeneration.
AsNeptuneBlockcontainsanon-mammalianproteinblockingagent,itisantigenicityforeigntomostmammalianimmunesystems.Inantigen-downELISAformats,thisreducesthepossibilityoffalse-positivesgeneratedfromendogenousantibodiesinthesamplereactingwithplateblockingproteins.NeptuneBlockisparticularlyusefulwhenworkingwithhuman,swine,andbovineserumsamples,asthereislittleinteractionwiththeblockingmoleculesresultinginlowerbackgrounds.
RecommendedUses:
- Antigen-downELISAs.
- SandwichELISAswithhighbackgrounds.
- ELISAstestinghumanandothermammaliansamples
