Type:MultiplexProteinArray
SpeciesReactivity:H
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;AllCorrectlyFolded,FunctionalProteins
Each75mmx25mmglassslideisspottedwith4identicalproteinarrays(alsocalled “subarrays”),eachwith 1631correctlyfoldedandfunctionalspottedproteins! Eachproteinisattachedtothestreptavidin-coatedslideexclusivelyviaabiotinylatedaffinitytag,specificallythebiotincarboxylcarrierprotein(BCCP)domainoftheE.coliacetylCoAcarboxylase.ThisaffinitytagwaschosenbecausetheBCCPdomainmustbefoldedintoitsnative3-dimensionalstructureinordertobecomebiotinylated