

Type:Kit
Applications:ApoptosisDetection
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:All
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All
PolycaspaseandCaspaseinvitroapoptosiskitsmakeiteasytomeasurecelldeathandapoptosisinculturedneuronsandmanyothercelltypes.Justaddthereagenttothemedia,letitincubate1-4hours,washthecellsandreadwithafluorescenceplatereader,microscope,orflowcytometer. Apoptoticcellsfluoresceredorgreen.
Polycaspase,Green-Publications
Image:PolycaspaseKit,Greeninaction.4outof5cellsareapoptotic:Jurkatcellswerelabeledthekit.4cellsfluorescegreen(left),butthegreyimage(right)reveals5cellsinthefield.The4greencellsareapoptotic=80%ofcellsinthisexperimenthadactivecaspases.