品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
商品描述
Type:Kit
Applications:ApoptosisDetection
E=ELISA;FACS;FC=FlowCytometry;FPLC=FastProteinLiquidChromatography;GF=GravityFlow;HPLC=HighPerformanceLiquidChromatography;ICC=Immunocytochemistry;IF=Immunofluorescence;IHC=Immunohistochemistry;IP=Immunoprecipitation;NAC=Non-adherentCellAssays;NB=NeutralizationofBioactivity;SE=SandwichELISA;TPE=TargetedProteinExpression;WB=Westernblotting;;AC=AdherentCellAssays;FM=FluorescentMicsroscopy;;;BSC-CM5=BiacoreSensorChipCM5;BSM=BiosactiveSmallMoleculeorPeptide;CDM=CellDifferentiationMedia;;;;;;HealthandFitness;;;DNAExtraction/Purification;;InvivoLikeAssaysSpeciesReactivity:All
B=Bovine;Ca=Cat;Ch=Chicken;D=Dog;EQ=Equine;GP=GuineaPig;H=Human;M=Mouse;P=Porcine;Pr=Primate;R=Rat;Rb=Rabbit;Y=Yeast;Xe=Xenopus;Ze=Zebrafish;;;;NA-NotApplicable;STP=Step-TactinProteins;All
Forgetthelysates.Assayforapoptosisviacaspaseactivityinwhole,livingcellswiththeFLICA®660PolycaspaseAssayKit:
- Ex:660nm/Em:680-690nm
- Wholecellanalysisviaflowcytometryorfluorescencemicroscopy
- FlexIBLemultiplexingwithadditionaldyesorprobes
- CompatibilitywithGFPandgreenautofluorescence
- Benchtopflowcompatible
Images: THP-1cellsweretreatedwitheitheraplaceboorPMAandLPS.Aftertreatmentandtrypsinization,FLICA660-YVAD-FMKfar-redcaspase-1reagentwasaddedtotheculturemedia.Flowcytometricanalysisofthepositivecontrolsample(top)revealsaslightincreaseincellsbearingactivecaspase-1enzymewhencomparedtothepopulationofplacebo-treatedcells(bottom).An8:1differentialwasachievedwiththepositivecontroltreatment.
thecreationoftheinflammasomeduringhostresponsestopathogensleadstotheactivationofcaspase1.Inflammation-relateddiseasemodelshaveillustratedaroleforcaspase1inasthma,rheumatoidarthritis,multiplesclerosisandotherdisorders(1,2).FAQs:FLICA®Caspase1InflammationandApoptosisDetectionKit
| Specifications | |
Target: | Caspase1 |
Excitation/Emission: | 660nm/680-690nm |
MethodofAnalysis: | FlowCytometer,FluorescenceMicroscope |
TypesofSamples: | cellculture,tissue |
KitContents: |
|
Storage: | 2°-8°C,ShipsOvernight(Domestic),InternationalPriorityShipping |
1.McIntire,C.etal,(2009)Inflammasomesininfectionandinflammation.Apoptosis14:522-35
2.Franchi,L.etal.(2009)Theinflammasome:acaspase-1-activationplatformthatregulatesimmuneresponsesanddiseasepathogenesis.NatureImmunol.10:241-47
