CellCulturesareusedeverydayasapartofresearchmethods.Yourelyontheseculturestobehealthy,yetupto35%maybecontaminatedduetomycoplasma.Mycoplasmacontaminationaffectsmanycellfunctionsincludingmetabolism,morphology,proteinsynthesisandcellproliferation-allofwhichleadtounreliableresultsaswellaslosttimeandresources.SincecontaminationisnottypicallyvisIBLebyturbidity,routinescreeningformycoplasmacontaminationisessential.
ThePCRMycoplasmaTestKitisadequatetodiagnosecellculturesinfectedwithmycoplasmas. Infectionsusuallyresultinmycoplasmatitersof105–108CFU/mL
ThekitallowsdetectionofAcholeplasmaandSpiroplasmaandthefollowingMycoplasmaspecies:
- M.fermentans
- M.hyorhinis
- M.arginini
- M.orale
- M.salivarium
- M.hominis
- M.pulmonis
- M.arthritidis
- M.bovis
- M.pneumoniae
- M.pirum
- M.capricolum
Sensitivity
TypeofMycoplasma | MinimumConcentrationDetected |
M.FERMENTANS | 240CFU/mL |
M.CAPRICOLOM | 110CFU/mL |
PENETRANS | 200CFU/mL |
HYORHINIS | 210CRU/mL |