ProteinG-purifiedrabbitanti-humanADAMTS-5polyclonalIgG.
ADAMTS(adisintegrinandmetalloproteinasewiththrombospondinmotif)aremultidomainextracellularproteinases,containingatleastonethrombospondintype-1repeat[1].Theenzymesareinvolvedinprocessingofextracellularmatrixproteinsandproteinsinthecirculation.ADAMTS5wasfirstpurifiedfrombovinenasalcartilageconditionedmediaandhumanADAMTS5CDNAwasclonedfromahumanlivercDNAlibrary[2].ADAMTS5consistsofaprodomain,acatalyticdomain,adisintegrindomain,athrombospondintype-1motif,aCys-richregionfollowedbyaspacersequenceandthrombospondinsubmotifs.Theprodomainismostlikelycleaved-offbyafurin-typeenzymebeforeADAMTS5isreleasedfromcells.
ADAMTS5isexpressedconstitutivelyinsynovium[3]anditdegradesaggrecan,themajorproteoglycanofarticularcartilage[4].LikeADAMTS4,anothermemberoftheADAMTS-family,ADAMTS5cleavesaggrecanat5sites[4].Fourcleavagesitesarelocatedinthechondroitinsulfate-richregionbetweenaggrecanglobulardomainsG2andG3,whileonesiteiscontainedintherod-shapedpolypeptidebetweenglobulardomainsG1andG2.Inaddition,ADAMTS5cleavesonemoresiteinthechondroitinsulfate-richregionthatisnotcleavedbyADAMTS4[4].ADAMTS5isinhibitedbyTIMP3[5,6]and2-macroglobulin[7].
Characteristics:ThepolyclonalantibodywasraisedagainstanADAMTS-5peptidederivedfromthehingeregionbetweenADAMTS-5disintegrin-andthrombospondin-likedomain.Forimmunizationapeptide-KLH-conjugatewasused.
Specificity:TheantibodyprimarilyreactswithADAMTS-5.WhenADAMTS-5andADAMTS-4areanalyzedwiththeantibodyinWesternblotsandELISA,ADAMTS-5isdetectedat50-foldlowerconcentrationthanADAMTS-4.
Concentration:100μg/100μl
Format:Thepackageis100μgantibodyin100μlphosphatebufferedsaline(20mMNa-phosphate,pH7.5,150mMNaCl)containing0.02%thimerosal.
Application:WesternblottingwithECL-detection
Storage:Theantibodyisstableuntiltheexpirydatewhenstoredat-70°C.Afterthawingitcanbekeptat2-8°Cformorethan3months.Repeatedfreezingandthawingshouldbeavoided.
