Specifications
When initiating studies with a particular E3 ligase, UBE2D3 (UbcH5c) is often selected due to the reported promiscuity of this E2. However, it should be noted that several E3 ligases (including Parkin) have been reported not to work with UBE2D3. UBE2L3 (UbcH7) has been shown to function with several E3 ligases, including E6-AP and Parkin. UBE2N/UBE2V2 functions as a heterodimeric E2 enzyme for the production of K63-linked ubiquitin chains, and is a good choice if you know that the E3 of interest builds this linkage type. This information can be a starting point in matching E2/E3 enzyme pairs for your ligase assays, but comprehensive profiling is still sorely needed.
The E2 Selection Panel is designed to provide the most flexible and cost-effective solution to this daunting task. In a single package, four* E2 conjugation enzymes from our catalog can be selected**. These enzymes can be used as stand alone products, or in conjugation with both the E3LITE Customizable Ubiquitin Ligase Kit or the E2 Profiling Kit.
*To order more than four E2s, please inquire about the price.**Several E2s are available with His6SUMO tag or without tag.***Appropriate datasheets for selected E2 enzymes will be included with purchase This product is patented. Please click here to learn more.
Info
Panel Contents Each E2 Selection Panel comes with (4) E2 Conjugating Enzymes (0.8 nmol each)Storage All components should be stored at -80°CStability All components are stable for 6 months under recommended storage conditions
Life Sensors的泛素和泛素链泛素是一种小的多肽，通过其C端与目标蛋白上的赖氨酸的ε-氨基缀合。这种缀合称为单泛素化。随后，可以利用遍在蛋白表面上的七个赖氨酸残基（K6，K11，K27，K29，K33，K48，K63）中的任何一个，将额外的遍在蛋白部分与初始遍在蛋白结合。这种泛素链形成称为多泛素化。泛素的酶结合是通过一系列酶进行的，即泛素激活酶E1，泛素结合酶E2和泛素连接酶E3。蛋白质的泛素化在细胞中是可逆的。单泛素化和多泛素化的链均被去泛素化酶催化的水解作用裂解 （DUB）。LifeSensors在遍在泛素途径的所有水平的酶（包括E1，E2和E3酶和DUB）上都有丰富的经验。LifeSensors对蛋白质纯化的广泛了解使我们能够生产大约30种E2酶，20种E3酶和35个DUB，并具有定制表达的能力。此外，我们广泛的检测方法简化了确认酶活性和确定E2-E3对兼容性的过程，并确保您可以选择最能解决希望探索的特定，关键生物学问题的酶。LifeSensors已为您的研究开发了多种泛素衍生物，包括用于结合相互作用研究的DUB耐药性聚泛素链，用于DUB分析和筛选的泛素结合物（荧光团和报告酶），选择性泛素链（二，三和四环素）泛素链）用于DUB特异性和结合测定，位点特异性赖氨酸泛素突变体用于E3连接酶和DUB选择性测定。
