Specifications
Hrd1isaRINGdomainE3ligasethatisinvolvedintheconjugationofubiquitintotargetsubstrates.Hrd1hasbeendemonstratedtofunctionwiththeE2enzymesUBE2D3(UbcH5c)andUBE2G2invitro.Hrd1isanER-associatedligaseinvolvedinERADandhasbeenlinkedtorheumatoidarthritis.Itispresentinhumanrheumatoidsynovialcellsandfoundtobeacausativefactorforarthropathyininvivostudies.Hrd1isalsofoundinbrainneuronsanditsproperregulationmaybelinkedtoneurodegenerativediseases.ThisconstructisaHrd1N-terminaldeletioncontainingresidues236-617consistingofthecompleteC-terminalcytoplasmicportion.TheN-terminaltransmembranespanningportionhasbeendeleted.ThisHrd1N-terminaldeletionmutantisactiveasanE3ligasecontainingtheRINGandsubstraterecognitiondomains.Hrd1iscurrentlymade-to-orderandrequiresa2-3weekleadtime.
Info
MolecularWeight | Cytoplasmicfragment(236-617):39.3kDa(withoutSUMOtag) |
Quantity | 25μg |
PhysicalState | Liquid |
Buffer | BufferedSalinewith10%glycerol |
Source | Humanrecombinantexpressedinbacteria |
Tag | SUMO |
Activity | Typicalenzymeconcentrationof20-1000nMisusedforinvitroconjugationdependingonassayconditions |
Storage | -80°C.Avoidrepeatedfreeze/thawcycles |