Specifications
IQFDiUbTechnologyLifeSensors’diubiquitinsubstratesrepresentanewclassofsubstratesforthecontinuousfluorescentmeasurementoftrueisopeptidaseactivity.TheC-terminusofwild-typeubiquitinisconjugatedviaanisopeptidebondtolysine11,48,or63ofasecondubiquitinmoleculewiththeresultantdiubiquitinforminganinternallyquenchedfluorescentFRETpair(IQF).Eachubiquitinislabeledwithasinglemoleculeofeitherafluorescentreporter(i.e.TAMRA)orahighlyefficientquenchingdye.CleavageoftheIQFDiUbbydeubiquitylasesleadstoseparationofthefluorophorefromquencherandsubsequentincreaseinobservedfluorescence.Theubiquitin-TAMRAreferencestandardcanbeusedtooptimizeandcalibratetheperformanceofindividualplatereaders(orfluorometers)formeasurementoftheratesofhydrolysisofourIQF-DiUbbyDUBs.
Info
Amount | 25µg |
MW | 9,161Da |
Concentration | 40µM |
Purity | >=90% |
Buffer | 50mMSodiumMES,pH6.0 |