Specifications

SUMO (Small Ubiquitin-related Modifier) is an ubiquitin-like family member that regulates a wide range of key cellular events. Sumoylation of proteins alters their intracellular localization, stability and interaction with other proteins. SUMO is conjugated to its substrates utilizing a cascade of events involving activation with E1 enzyme (SAE1/SAE2), conjugation, involving the E2 enzyme (UBC9) and substrate modification, through the cooperation of the E2 and E3 protein ligases. SUMO and Nedd8 pathways utilize a single E1 and a single E2 in combination with a few known E3s. The dimeric activating enzyme E1 utilizes ATP to adenylate the C-terminal glycine residue of all SUMO proteins, forming a high- energy thiolester bond with the cysteine residue of SAE2.SUMO E1 enzyme is a heterodimer of His-tagged SAE1 and un-tagged SAE2.

Info

Purity: >80%Molecular Weight: 39 kDa and 73 kDaQuantity: 25ugPhysical State: Liquid Buffer: 20mM Tris, 150 mM NaCl, 2 mM βME, 10% glycerolSource: Human recombinant enzyme purified from E.coliTag: His6-tagged SAE1 and untagged SAE2Activity: 100nM is used for in vitro conjugationStorage: -80o C. Avoid repeated freeze/thaw cycles

Life Sensors的泛素和泛素链泛素是一种小的多肽，通过其C端与目标蛋白上的赖氨酸的ε-氨基缀合。这种缀合称为单泛素化。随后，可以利用遍在蛋白表面上的七个赖氨酸残基（K6，K11，K27，K29，K33，K48，K63）中的任何一个，将额外的遍在蛋白部分与初始遍在蛋白结合。这种泛素链形成称为多泛素化。泛素的酶结合是通过一系列酶进行的，即泛素激活酶E1，泛素结合酶E2和泛素连接酶E3。蛋白质的泛素化在细胞中是可逆的。单泛素化和多泛素化的链均被去泛素化酶催化的水解作用裂解 （DUB）。LifeSensors在遍在泛素途径的所有水平的酶（包括E1，E2和E3酶和DUB）上都有丰富的经验。LifeSensors对蛋白质纯化的广泛了解使我们能够生产大约30种E2酶，20种E3酶和35个DUB，并具有定制表达的能力。此外，我们广泛的检测方法简化了确认酶活性和确定E2-E3对兼容性的过程，并确保您可以选择最能解决希望探索的特定，关键生物学问题的酶。LifeSensors已为您的研究开发了多种泛素衍生物，包括用于结合相互作用研究的DUB耐药性聚泛素链，用于DUB分析和筛选的泛素结合物（荧光团和报告酶），选择性泛素链（二，三和四环素）泛素链）用于DUB特异性和结合测定，位点特异性赖氨酸泛素突变体用于E3连接酶和DUB选择性测定。