TheE3LITECustomizableUbiquitinLigaseKitisflexIBLebydesign,essentiallyprovidingasingularplatformforthefocusedinvestigationofanyE2/E3enzymepair,aswellasanyparticularpolyubiquitinlinkagetype.Insupportofthisflexibleplatform,LifeSensorsoffersthewidestarrayofreagentsonthemarkettomeetyourresearchneeds.Thekititselfcanbeassembledwithanyoneofmorethantwenty-fourE2conjugationenzymes,aselectionrepresentingmembersfromeachenzymeclass.FormoreE2enzymes,assembleapanelof(4)fromE2Selectionpanel.Therecombinantubiquitincomponentofthiskitcanbewild-type,variantsthatspecificallyformK48linkagetypes,orvariantsthatformlinkagesonlythroughoneofthesevenpossiblesurfaceexposedlysines.
*Platesmustbeusedwithin30daysofreceipt.
ThisproductisprotectedbyoneormoreUSorForeignpatents.Pleasereadthe LimitedUseLabelLicense tolearnmore.Bypurchasingthisproduct,thepurchaseragreestocomplytotheseterms.
PriceshownisfortheoneE2enzymeincludedinthekit.Additional(4)E2panelcanbepurchasedfor$195.ToordermoreE2s,pleaseinquireabouttheprice. Email info@lifesensors.com formoreinformation,includingcommercialavailABIlity.
Info
KitContents:
- UbiquitinE1ActivatingEnzyme
- ChoiceofUbiquitinE2ConjugatingEnzyme(s)
- E1-E2-E3Control
- ChoiceofRecombinantUbiquitin
- DetectionReagent1
- LinearPolyubiquitinControl
- (2)modular96-wellplatescoatedwithLifeSensorsproprietaryreagent
| Storage | Allcomponentsshouldbestoredat-80°C. |
| Stability | Allcomponents,exceptformicrotiterplates,arestableforatleast2monthswhenstoredat-80°C. Platesmustbeusedwithin30days. |
Theselectionofappropriatestreptavidinreagentiscriticaltogeneratingarobustsignal.Wedonotrecommendusingavidin-horserADIshperoxidase(HRP)conjugates.
Applications
- QuantitativeAnalysis of UbiquitinE2ConjugationorE3LigaseActivity
- DemonstrationofnovelE2orE3Activity
- HighThroughputScreening(HTS)ofantagoNISToragonistsofligaseactivity
- Monitorenzymeactivityduringpurification
Benefits
- MonitorE3activityinsolutionphase,withtheE2ofyourchoice
- Plate-basedformatisamenabletohigh-throughputscreening(HTS)
- DetectionsystemprovidesrobustreadoutforE3ligaseactivity
- E3LITEassayutilizesnon-radioactivereportersubstrates
- E3LITEassaydoesnotrequireexcitationintheUVrange(reducingfalsepositiverate)
Documents
Life Sensors的泛素和泛素链泛素是一种小的多肽，通过其C端与目标蛋白上的赖氨酸的ε-氨基缀合。这种缀合称为单泛素化。随后，可以利用遍在蛋白表面上的七个赖氨酸残基（K6，K11，K27，K29，K33，K48，K63）中的任何一个，将额外的遍在蛋白部分与初始遍在蛋白结合。这种泛素链形成称为多泛素化。泛素的酶结合是通过一系列酶进行的，即泛素激活酶E1，泛素结合酶E2和泛素连接酶E3。蛋白质的泛素化在细胞中是可逆的。单泛素化和多泛素化的链均被去泛素化酶催化的水解作用裂解 （DUB）。LifeSensors在遍在泛素途径的所有水平的酶（包括E1，E2和E3酶和DUB）上都有丰富的经验。LifeSensors对蛋白质纯化的广泛了解使我们能够生产大约30种E2酶，20种E3酶和35个DUB，并具有定制表达的能力。此外，我们广泛的检测方法简化了确认酶活性和确定E2-E3对兼容性的过程，并确保您可以选择最能解决希望探索的特定，关键生物学问题的酶。LifeSensors已为您的研究开发了多种泛素衍生物，包括用于结合相互作用研究的DUB耐药性聚泛素链，用于DUB分析和筛选的泛素结合物（荧光团和报告酶），选择性泛素链（二，三和四环素）泛素链）用于DUB特异性和结合测定，位点特异性赖氨酸泛素突变体用于E3连接酶和DUB选择性测定。
