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蚂蚁淘在线 / 品牌中心 / Life Sensors / LifeSensors/UM400M- 1mL Anti-Ub Control Agarose Magnetic Beads (negative control)/UM400M- 1mL Slurry Anti-Ub control Magneti
商品描述
Description
Foruse in “pre-clearing” cell lysates, as well as a negative control for thepulldown of polyubiquitylated proteins in conjunction with magnetic beads-conjugatedTUBEs (UM401M and UM402M).
Generalrecommendations: Pre-clear or use as a negative control for 1ml of cell lysate(3-5 mg/ml total protein) through incubation with 100µl of slurry for 1hr(4°C). Individual conditions may have to be optimized by the end user. Controlmagnetic beads is generated by blocking an activated matrix (identical to thatused for TUBEs conjugation) with ethanolamine. Control magnetic beads (1 mL) issupplied as a slurry in PBS, pH 7.2.
Info
Storage: +4°C. Storage below this temperature may result in freezing and/or loss of function. |
Applications
- Negative control for pull-down experiments
- "Pre-clearing" of cell lysates prior to pull-down experiments
Documents
- Documents
