Antigen/Purification: Collapse
The antigen is synthetic peptide from the N-terminal region of rat FTO.
The antibody is Protein G purified culture supernatant.
Biological Significance: Collapse
The FTO gene is the most robust gene for common obesity characterized to date. FTO gene expression has been found to be significantly upregulated in the hypothalamus of rats after food deprivation and strongly negatively correlated with the expression of orexin peptide which is involved in the stimulation of food intake (Fredricksson R et al., 2008). Deletion analysis of FTO gene in mice showed that Fto is functionally involved in the control of both energy intake and energy expenditure (Fischer J et al., 2009).
Storage
100 µl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
For long term storage –20° C is recommended in undiluted aliquots. Stable at –20° C for up to one year after date of receipt. Avoid freeze/thaw cycles.
Product Specific References
Guan, B.J., van Hoef, V., Jobava, R., Elroy-Stein, O., Valasek, L.S., Cargnello, M., Gao, X.H., Krokowski, D., Merrick, W.C., Kimball, S.R. and Komar, A.A., 2017. A Unique ISR Program Determines Cellular Responses to Chronic Stress. Molecular Cell, 68(5), pp.885-900. PMID: 29220654
Yu, J., Chen, M., Huang, H., Zhu, J., Song, H., Zhu, J., Park, J. and Ji, S.J., 2017. Dynamic m6A modification regulates local translation of mRNA in axons. Nucleic Acids Research. Nov 23. PMID: 29186567
Zhou, J., Wan, J., Gao, X., Zhang, X., Jaffrey, S. R., & Qian, S. B. (2015). Dynamic m6A mRNA methylation directs translational control of heat shock response. Nature, 526(7574), 591-594. PMID: 26458103
Gulati, P., Avezov, E., Ma, M., Antrobus, R., Lehner, P., O’Rahilly, S., & Yeo, G. S. (2014). Fat mass and obesity-related (FTO) shuttles between the nucleus and cytoplasm. Bioscience reports, 34(5), 621-628. PMID: 25242086
Gulati P, Cheung MK, Antrobus R, Church CD, Harding HP, Tung YC, Rimmington D, Ma M, Ron D, Lehner PJ, Ashcroft FM, Cox RD, Coll AP, O’Rahilly S, Yeo GS (2013) Role for the obesity-related FTO gene in the cellular sensing of amino acids. Proc Natl Acad Sci U S A. Jan 28. PMID: 23359686
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