Theantibodyproducesmoderatelabelingofrapheneuronsinnormalrat.InratswhoseSEROtonergicsystemhasbeenactivated,stainingintensityisincreasedtoamaximumlabel.Recommendeddilutionoftheantiserumis1/4000-1/8000forbiotin-streptavidin/HRPtechnique.

Thespecificityoftheantiserumwasevaluatedusingamodelsystemofgelatin-indoleplugsbyamethodsimilartopublishedprocedures(SchipperandTilders,1983).Resultsshowedthatthe5-HIAAantibodydosedependentlystained5-HIAAbutdidnotstainanyconcentrationof5-HTor5-HTP.Theantiserumwasalsotestedbypre-adsorptionat25µg/mLwithvariousBSAconjugates.Whilepre-adsorptionwith5-HIAAconjugatecompletelyeliminatesimmunolabeling,pre-adsorptionwithconjugatesof5-HT,5-HTPanddopaminehadnoeffectonstainingintensityordistributionofstain.

PhotoDescription:IHCimageofneuronsstainingfor5-H1AAintheraphenucleusoftheratbrainstem.Thetissuewasfixedwith4%formaldehydeinphosphatebuffer,beforebeingremovedandpreparedforvibratomesectioning.FloatingsectionswereincubatedatRTin10%goatseruminPBS,beforestandardIHCprocedure.Primaryantibodywasincubatedat1:5000for48hours,goatanti-rabbitsecondarywassubsequentlyaddedfor1hourafterwashingwithPBS.Lightmicroscopystainingwasachievedwithstandardbiotin-streptavidin/HRPprocedureandDABchromogen. 

Host:Rabbit

Quantity/Volume:100µL

State:LyophilizedWholeSerum

ReactsWith::Rat,TritoniaDiomedea(SeaSlug)

AlternateNames:anti-5-HIAA

RRID:AB_572208

Immunogen:5-HIAA

GeneSymbol:SLC6A4

EntrezGeneID:6532

NCBIGeneAliases:5-HTT,5-HTTLPR,5HTT,HTT,OCD1,SERT,hSER