Cytochrome c Apoptosis Assay Kit.

Kit Summary:

• Detection method- Western blotting

• Sample type- Cells and Tissues

• Species reactivity- Mammalian

• Applications- Effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides an easy-to-use procedure and reagents for separating a mitochondria-enriched fraction from cytosol.

Features & Benefits:

• Simple procedure; takes ~3 hours

• Fast and convenient

• Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit. The procedure is simple and straightforward. No ultracentrifugation is required.

Kit Components:

• Mitochondria Extraction Buffer

• 5X Cytosol Extraction Buffer

• DTT (1 M)

• 500X Protease Inhibitor Cocktail

• Anti-Cytochrome c mouse mAb (0.2 mg/ml) .

Description:

Cytochrome c plays an important role in apoptosis. The protein is located in the space between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c from the mitochondria into cytosol where it binds to Apaf-1. The cytochrome c/Apaf-1 complex activates caspase-9, which then activates caspase-3 and other downstream caspases. Cytochrome c Releasing Apoptosis Assay Kit provides an effective means for detecting cytochrome c translocation from mitochondria into cytosol during apoptosis. The kit provides unique formulations of reagents to isolate a highly enriched mitochondria fraction from cytosol. The procedure is so simple and easy to perform, no ultracentrifugation is required and no toxic chemicals are involved. Cytochrome c releasing from mitochondria into cytosol is then determined by Western blotting using the cytochrome c antibody provided in the kit.

Storage Conditions: -20°C.

Shipping Conditions: gel pack.

USAGE: For Research Use Only! Not For Use in Humans.

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