Trypsin Activity Assay Kit - 100 reactions

Kit Summary:

• Detection method - Absorbance (405 nm).

• Sample type - Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc.

• Species reactivity - Mammalian.

• Application - The kit detects 10-100 mU (p-NA unit) trypsin in various samples.

Features & Benefits:

• Simple procedure; takes ~ 1-2 hours based on the levels of trypsin.

• Fast and convenient.

Kit components:

• Trypsin Assay Buffer.

• Trypsin Substrate (in DMSO).

• Positive Control (~2U, lyophilized).

• p-NA Standard (2 mM).

• Trypsin Inhibitor (TLCK, 20 mM).

• Chymotrypsin Inhibitor (TPCK,10 mM).

Description:

Trypsin (EC 3.4.21.4) is a serine protease found in the digestive system of many vertebrates, where it hydrolyses proteins. Trypsin is produced in the pancreas as the inactive proenzyme trypsinogen. Active trypsin predominantly cleaves peptide chains at the carboxyl side of the amino acids lysine or arginine, except when either is followed by proline. It is used for numerous biotechnological processes. In the assay, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at λ=405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured. The kit detects 10-100 mU (p-NA unit) trypsin in various samples.

Storage Conditions: -20°C.

Shipping Conditions: gel pack.

USAGE: For Research Use Only! Not For Use in Humans.

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