Mitochondrial DNA Isolation Kit - 50 reactions

Kit Summary:

• Sample type - Cell culture (adherent and suspension), fresh and frozen tissues.

• Species reactivity - Mammalian.

• Applications - Enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications.

Features & Benefits:

• Simple procedure; takes less than 2 hours.

• Fast and convenient.

• The kit provides unique formulations of buffers and reagents for isolating a highly enriched mitochondrial fraction. Mitochondrial DNA are then isolated using reagents included in the kit.

Kit components:

• 5X Cytosol Extraction Buffer.

• Mitochondrial Lysis Buffer.

• Enzyme B Mix (lyophilized).

• TE Buffer.

Description:

Mitochondria are semiautonomous organelles which functions in aging process, apoptosis, anti-HIV drugs, and cancers. Mitochondrial DNA (mtDNA) has a very high mutation rate and the mutations on mtDNA appear to be related to certain diseases such as diabetes, Alzheimer’s disease, and muscle disorders. Isolation and quantification of mtDNA are often required to study the relationships between the diseases and mtDNA. The Mitochondrial DNA Extraction Kit provides convenient tools for isolating mtDNA from a variety of cells and tissues in high yield and purity, without contaminations from genomic DNA. The purified mtDNA can be used for a variety of studies such as enzyme manipulations, Southern blotting, cloning, PCR analysis, and amplifications.

Storage Conditions: -20°C.

Shipping Conditions: gel pack.

USAGE: For Research Use Only! Not For Use in Humans.

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