Myeloperoxidase (MPO) Fluorometric Activity Assay Kit.

Highly Sensitive Assay, HTS

Kit Summary:

• Detection method- Fluorescence (Ex/Em = 485/525 nm)

• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids etc.

• Species reactivity- Mammalian.

Features & Benefits:

• Simple procedure

• Fast and convenient

• Sensitive assays for measuring MPO as low as 0.5 μU per well.

Kit Components:

• Assay Buffer

• MPO Substrate Stock

• MPO Probe

• Fluorescein Standard (1 mM)

• MPO Positive Control

Description:

Myeloperoxidase (MPO) is a peroxidase enzyme (EC 1.11.1.7) most abundantly present in neutrophil granulocytes. It is a green hemoprotein found in neutrophils and monocytes that catalyzes the reaction of hydrogen peroxide and halide ions to form cytotoxic acids and other intermediates that play a role in the oxygen-dependent killing of tumor cells and microorganisms. Its heme pigment causes the green color in secretions rich in neutrophils, such as pus and some forms of mucus. Furthermore, it can oxidize tyrosine to a tyrosyl radical using hydrogen peroxide as an oxidizing agent. In the MPO Assay Kit, MPO catalyzes the production of NaClO from H₂O₂ and NaCl. Subsequently, NaClO will react stoichiometrically with Aminophenyl fluorescein (APF) to generate fluorescein, which has a strong fluorescence and can be detected at Ex/Em = 485/525 nm. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput assay of MPO activity. This kit can be used to detect MPO activity as low as 0.5 μU per well.

Storage Conditions: -20°C.

Shipping Conditions: gel pack.

USAGE: For Research Use Only! Not For Use in Humans.

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