Product Highlights:
The TruSeq Rapid Exome Library Prep Kit delivers a simple, efficient method for high-confidence calling of exonic variants. The fast library preparation and exome enrichment workflow deliver libraries in 1 day with no need for additional equipment, giving labs the flexibility to plan projects according to sample volume. This kit offers:
- Enhanced transposome chemistry results in reduced bias, providing consistent library preparation and exome enrichment.
- Rapid, automation-friendly workflow completes in 1 day with only 3 hours of hands-on time.
- High coverage uniformity across exonic regions and on-target %
Proven TruSeq Data Quality
The TruSeq Rapid Exome Library Prep Kit delivers an average of 75% of on-target sequencing reads. This high on-target percentage requires fewer sequencing cycles to reach desired coverage levels, but still achieves uniform coverage for high-confidence results.
To learn more about calculating coverage estimates, see the sequencing coverage calculator.
Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this library preparation kit
Specifications:
Assay Time | 1 day |
Hands-On Time | 3 hours |
Input Quantity | 50 ng genomic DNA |
Content Specifications | Captures coding exons. Covers 45 Mb of exonic content (≥98% of RefSeq, CCDS, and Ensembl coding content). |
Mechanism of Action | Transposase-based fragmentation and exome enrichment with biotinylated capture probes |
Multiplexing | Up to 12-plex enrichment |
Technology | Sequencing |
Species Category | Human |
Automation Capability | Liquid Handling Robots |
System Compatibility | NextSeq 500,NovaSeq 6000,HiSeq 4000 |
Method | Exome Sequencing |
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