Product Highlights:
Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex capability and a simplified workflow with master-mixed reagents.
Kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples (including up to 384 RNA-Seq samples on a single HiSeq 2500 System run).
Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with the easiest-to-use sample preparation workflow offered by any NGS platform.
Find an up-to-date list of high-throughput automation vendors with robotic systems compatible with this library preparation kit
Specifications:
| Assay Time | ~10.5 hours |
| Hands-On Time | ~4.5 hours |
| Input Quantity | 0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails) |
| Content Specifications | Captures the coding transcriptome (without strand information) |
| Mechanism of Action | Oligo-dT beads capture polyA tails |
| Multiplexing | Up to 24-plex per lane |
| System Compatibility | Genome Analyzer IIx,HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000 |
| Species Category | Other,Mammalian,Bovine,Mouse,Human,Rat |
| Variant Class | Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants |
| Technology | Sequencing |
| Method | mRNA Sequencing |
| Automation Capability | Liquid Handling Robots |
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