DBCO PEG acid, MW 1000, 2000, 3400, 5000, 10000 Da:
Nanocs’ DBCO (dibenzocyclooctyne) PEG acid, DBCO-PEG-COOH, is a heterobifunctional reactive PEG derivative that can go Click Chemistry reaction without a need of any metal catalysts. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free click reaction, is a bioorthogonal reaction that enables the conjugation of two molecules in aqueous solution. DBCO PEG derivatives possess fast kinetics and stability in aqueous buffer. DBCO reagents can be used to label azide-modified biomolecules spontaneously without the need for toxic Cu catalysts. Carboxylic acid group, on the other hand, can be activated to react with amines, hydroxy and other functional groups.
Nanocs DBCO PEG acid features:
- Appearance: Off-white solid or semi-solid depends on MW of PEG;
- Solubility: Soluble in water, DMSO and chloroform;
- Reactivity: DBCO reactive to azide group while acid group reactive to amine and hydroxy groups.
Copper-free Click reaction procedures:
- Prepare the azide-containing reagents in reaction buffer.
- Add DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
- Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 40C.
- Purify conjugates either by dialysis or size-exclusion chromatograph.
Storage Conditions:
- Store at -200C, desiccate. Protect from light. Avoid frequent thaw and freeze.
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