Tissue Type: Skin Histology: Melanotic melanoma; Malignant melanoma; non epithelial Metastatic site: lymph node p53 Status: 1 ECAD Methylation: 34 MDR Function: 12 Institution: Norsk Hydro s Institute, Norway Contributor: Fodstad, O Reference; Int J Cancer. 41:442-449, 1988 LOX-IMVI is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification. The Transfection Reagent: LOX-IMVI Cell Avalanche Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting LOX-IMVI cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for LOX-IMVI cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche Cell type/cell line specific transfection reagent series? Features: Much less reagent needed for each transfection: 0.5 ml is able to transfect about 1000 wells of 24-well plate Deliver single or multiple plasmids Free of animal-derived components Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells Active in serum-containing medium Suitable for Reverse TransfectionCompatible with transfection in any plate format Economical: High efficiency means less amount of nucleic acid reagent is needed Developed and manufactured by EZ Biosystems FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on LOX-IMVI cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for LOX-IMVI cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this LOX-IMVI Cell Avalanche Transfection Reagent. LOX-IMVI Cell Avalanche Transfection Reagent (human melanoma cell) can also be used on the following cells with high transfection efficiencies. M14 Cell SK-MEL-2 Cell SK-MEL-5 Cell A-375 Cell SK-MEL-28 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. Orders, if entered prior to 4:00 pm Eastern Standard Time, may be shipped the same day (Monday through Thursday) via FedEx Standard/Priority Overnight for US domestic customers; the same day (Friday, Monday, Tuesday) via FedEx International Priority for International customers (deliver in about 2-4 days). The item will be shipped at ambient temperature. It will be good for 1 year after delivery if kept refrigerated.

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