Clone | TUB-2 |
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Size | 100μg/vial |
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Form | lyophilized |
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Igtype | mouseIgG1 |
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Specificity | Nocrossreactivitywithotherproteins. |
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Species | Human,mouse,rat |
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Immunogen | Tubulinfromratbrain. |
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Contents | Mouseascitesfluid,1.2%sodiumacetate,2mgBSA,with0.01mgNaN3as |
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preservative. |
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WesternblotAt2μg/mlwiththeappropriatesystemtodetectβ-Tubulinincellsandtissues.
Immunohistochemistry
At0.5-1μg/mltodetectβ-Tubulininformalinfixedandparaffinembeddedtissues.
(Paraffin-embeddedSection)
Otherapplicationshavenotbeentested.
Optimaldilutionsshouldbedeterminedbyendusers.
Reconstitution:1.2%sodiumacetateorneutralPBS.If1mlofPBSisused,theantibodyconcentrationwillbe100μg/ml.
Storage:At-20˚Cforoneyear.Afterreconstitution,at4˚Cforonemonth.Itcanalsobealiquottedandstoredfrozenat-20˚Cforalongertime.Avoidrepeatedfreezingandthawing.
1.Floyd-Smith,G.A.;deMartinville,B.;Francke,U.:Abeta-tubulinexpressedgeneM40(TUBB)islocatedonhumanchromosome6andtworelatedpseudogenesarelocatedonchromosomes8(TUBBP1)and13(TUBBP2).(Abstract)Cytogenet.CellGenet.40:629,1985.
2.Floyd-Smith,G.A.;deMartinville,B.;Francke,U.:Anexpressedbeta-tubulingene,TUBB,islocatedontheshortarmofhumanchromosome6andtworelatedsequencesaredispersedonchromosomes8and13.Exp.CellRes.163:539-548,1986.
3.Monzo,M.;Rosell,R.;Sanchez,J.J.;Lee,J.S.;O"Brate,A.;Gonzalez-Larriba,J.L.;Alberola,V.;Lorenzo,J.C.;Nunez,L.;Ro,J.Y.;Martin,C.:aclitaxelresistanceinnon-small-
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