SKU#: 2101241001
Lot No. 03C01
Ig type: rabbit IgG
Size: 100μg/vial
Specificity: Human, rat, mouse. No cross reactivity with other proteins.
Immunogen: A synthetic peptide corresponding to a sequence near the C-terminal of human Annexin I, different from the related rat and mouse sequence by two amino acids.
Purity: Immunogen affinity purified.
Applications:
Western blot: At 1-2μg/ml with the appropriate system to detect Annexin I in cells and tissues.
Immunohistochemistry (P): At 1-2μg/ml to detect Annexin I in formalin fixed and paraffin embedded tissues. Digesting the sections is required.
Immunocytochemistry: Suitable
Other applications have not been tested.
Optimal dilutions should be determined by end user.
Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg, Thimerosal, 0.05mg NaN3.
Reconstitution: 0.2ml of distilled water will yield a concentration of 500μg/ml.
At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for longer time.
1. Wallner, B. P.; Mattaliano, R. J.; Hession, C.; Cate, R. L.; Tizard, R.; Sinclair, L. K.; Foeller, C.; Chow,
E. P.; Browning, J. L.; Ramachandran, K. L.; Pepinsky, R. B. : Cloning and expression of human
lipocortin, a phospholipase A2 inhibitor with potential anti-inflammatory activity. Nature 320: 77-81,
1986. .
2. Kovacic RT, Tizard R, Cate RL, FreyAZ, Wallner
BP. Correlation of gene and protein structure of rat and human lipocortin I. Biochemistry. 1991 Sep
17;30(37):9015-21.
3. Walther, A.; Riehemann, K.; Gerke, V. A novel ligand of the formyl peptide receptor: annexin I
regulates neutrophil extravasation by interacting with the FPR. Molec. Cell 5: 831-840, 2000.
