SKU#:2100111001

LotNo.03A01

Igtype:rabbitIgG

Size:100μg/vial

Specificity:Human,mouse,rat.Nocrossreactivitywithotherproteins.

Immunogen:AsyntheticpeptidecorrespondingtoasequenceattheN-terminalofhumanALPL,differentfromtherelatedratandmousesequencebytwoaminoacids.

Purity:Immunogenaffinitypurified.

Applications:

Westernblot:At1-2μg/mlwiththeappropriatesystemtodetectALPLincellsandtissues.

Immunohistochemistry(P):At1-2μg/mltodetectALPLinformalinfixedandparaffinembeddedtissues.Boilingthesectionsisrequired.

Otherapplicationshavenotbeentested.

Optimaldilutionsshouldbedeterminedbyenduser.

Contents:Eachvialcontains5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mg,Thimerosal,0.05mgNaN3.

Reconstitution:0.2mlofdistilledwaterwillyieldaconcentrationof500μg/ml.

EAt-20˚Cforoneyear.Afterreconstitution,at4˚Cforonemonth.Itcanalsobealiquottedandstoredfrozenat-20˚Cforlongertime.

1.Fedde,K.N.;Whyte,M.P.:Alkalinephosphatase(tissue-nonspecificisoenzyme)isa

phosphoethanolamineandpyridoxal-5-prime-phosphateectophosphatase:normaland

hypophosphatasiafibroblaststudy.Am.J.Hum.Genet.47:767-775,1990.

2.Weiss,M.J.;Cole,D.E.C.;Ray,K.;Whyte,M.P.;Lafferty,M.A.;Mulivor,R.A.;Harris,H.:A

missensemutationinthehumanliver/bone/kidneyalkalinephosphatasegenecausingalethalformof

hypophosphatasia.Proc.Nat.Acad.Sci.85:7666-7669,1988.