SKU#:2100081001
LotNo.0131012022464
Igtype:rabbitIgG
Size:100μg/vial
Specificity:Human,rat.Nocrossreactivitywithotherproteins.
Immunogen:AsyntheticpeptidecorrespondingtoasequenceattheC-terminalofhumanActin(367-377aa),identicaltotherelatedratsequence.
Purity:Immunogenaffinitypurified.
Applications:
Concentration | TestedSpecies | Concludedspecies | AntigenRetrieval | |
WB | 1μg/ml | Hu,Rat | Ms | - |
IHC-P | 2μg/ml | Hu,Rat | Ms | ByHeat |
IHC-F | - | - | - | - |
ICC | - | - | - | - |
Otherapplicationshavenotbeentested.
Optimaldilutionsshouldbedeterminedbyenduser
Contents:Eachvialcontains5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mg,Thimerosal,0.05mgNaN3.
Reconstitution:0.2mlofdistilledwaterwillyieldaconcentrationof500μg/ml.
At-20˚Cforoneyear.Afterreconstitution,at4˚Cforonemonth.Itcanalsobealiquottedandstoredfrozenat-20˚Cforlongertime.
1.Gunning,P.,Ponte,P.,Okayama,H.,Engel,J.,Blau,H.andKedes,L.Isolationandcharacterizationof
full-lengthCDNAclonesforhumanalpha-,beta-,andgamma-actinmRNAs:skeletalbutnotcytoplasmic
actinshaveanamino-terminalcysteinethatissubsequentlyremoved.
Mol.Cell.Biol.1983;3(5),787-795.
2.Goebel,H.H.,Brockmann,K.,Bonnemann,C.G.,Warlo,I.A.,Hanefeld,F.,Labeit,S.,Durling,H.J.and
Laing,N.G.Actin-relatedmyopathywithoutanymissensemutationintheACTA1Gene.
J.ChildNeurol.2004;19(2),149-153.
3.Laing,N.G.,Clarke,N.F.,Dye,D.E.,Liyanage,K.,Walker,K.R.,Kobayashi,Y.,Shimakawa,S.,
Hagiwara,T.,Ouvrier,R.,Sparrow,J.C.,Nishino,I.,North,K.N.andNonaka,I.Actinmutationsareone
causeofcongenitalfibretypedisproportion.Ann.Neurol.2004;56(5),689-694.
