| Productname | PolyclonalAnti-ACTB |
| CatalogNo. | 2107661001 |
| Size | 100μg/vial |
| Form | lyophilized |
| Igtype | rabbitIgG |
| Immunogen | AsyntheticpeptidecorrespondingtoasequenceattheN-terminal |
| ofhumanβ-Actin,identicaltotherelatedratandmouse | |
| sequences. | |
| Purification | Immunogenaffinitypurified |
| Reconstitution | 0.2mlofdistilledwaterwillyieldaconcentrationof500μg/ml. |
| Application |
Concentration | TestedSpecies | ConcludedSpecies | AntigenRetrieval | |
WB | 0.1-0.5μg/ml | Hu,Rat | Ms | - |
Storeat-20°Cforoneyear.Afterreconstitution,storeat4°Cforonemonth.Itcanalsobealiquottedandstoredfrozenat-20°Cforalongertime.Avoidrepeatedfreezingandthawing.
1.Gunning,P.,Ponte,P.,Okayama,H.,Engel,J.,Blau,H.,Kedes,L.Isolationandcharacterizationoffull-lengthCDNAclonesforhumanalpha-,beta-,andgamma-actinmRNAs:skeletalbutnotcytoplasmicactinshaveanamino-terminalcysteinethatissubsequentlyremoved.Molec.Cell.Biol.3:787-795,1983.
2.Karakozova,M.,Kozak,M.,Wong,C.C.L.,Bailey,A.O.,Yates,J.R,III,Mogilner,A.,Zebroski,H.,Kashina,A.Arginylationofbeta-actinregulatesactinCytoskeletonandcellmotility.Science313:192-196,2006.
3.Ueyama,H.,Inazawa,J.,Nishino,H.,Ohkubo,I.,Miwa,T.FISHlocalizationofhumancytoplasmicactingenesACTBto7p22andACTG1to17q25andcharacterizationofrelatedpseudogenes.Cytogenet.CellGenet.74:221-224,1996.
