SKU#:2101171001
LotNo.03A01
Igtype:rabbitIgG
Size:100μg/vial
Specificity:Human,mouse,rat.Nocrossreactivitywithotherproteins.
Immunogen:AsyntheticpeptidecorrespondingtoasequenceattheC-terminalofhumanABI-1,differentfromtherelatedratandmousesequencebysingleaminoacid.
Purity:Immunogenaffinitypurified.
Applications:
Westernblot:At1-2μg/mlwiththeappropriatesystemtodetectABi-1incellsandtissues.
Immunohistochemistry(P):At0.5-1μg/mltodetectABi-1informalinfixedandparaffinembeddedtissues.
Immunohistochemistry(F)At0.5-1μg/mltodetectAbi-1informalinoracetonefixedtissues.
Immunocytochemistry:At0.5-1μg/mltodetectAbi-1inacetonefixedcell.AntigenretrievalbyPepsinandTrypsinisrequired.
Otherapplicationshavenotbeentested.
Optimaldilutionsshouldbedeterminedbyenduser.
Contents:Eachvialcontains5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mg,Thimerosal,0.05mgNaN3.
Reconstitution:0.2mlofdistilledwaterwillyieldaconcentrationof500μg/ml.
At-20˚Cforoneyear.Afterreconstitution,at4˚Cforonemonth.Itcanalsobealiquottedandstoredfrozenat-20˚Cforlongertime.
1.Tani,K.;Sato,S.;Sukezane,T.;Kojima,H.;Hirose,H.;Hanafusa,H.;Shishido,T.:Ablinteractor1
promotestyrosine296phosphorylationofmammalianEnabled(Mena)byc-Ablkinase.J.Biol.Chem.
278:21685-21692,2003.
2.Taki,T.;Shibuya,N.;Taniwaki,M.;Hanada,R.;Morishita,K.;Bessho,F.;Yanagisawa,M.;Hayashi,
Y.:ABI-1,ahumanhomologtomouseAbl-interactor1,fusestheMLLgeneinacutemyeloidleukemia
witht(10;11)(p11.2;q23).Blood92:1125-1130,1998.
