SampleTyple | Plasma,Serum,Cellculturesupernatant,otherbodyfluid | |||||||
Components | ||||||||
Method | Colorimetricmethodat570nm | |||||||
StandardCurve | ||||||||
Sensitivity | 10µM-500µM | |||||||
ReactionTime | 30minutes | |||||||
Applications | ForBIOLOGicalresearch: | |||||||
Storage | -80°C | |||||||
| Notes | Materialneededbutnotsupplied Aplatereadercapableofmeasuringabsorbanceat570nm AdjustablePipettesandarepeatpipettor Thedistilledwater(milliQorHPLC-grade) ClearFlatbottom96-wellplatesifnotincludedinthekitpurchased | |||||||
| Shipping | Icepacks | |||||||
ReagentPreparation Note:Allreagentsarefrozen.Werecommendyouspinsmallvialsbeforeopening. 1.UricAcidAssayBuffer(10x) PleasedonotdiluteintheprovidedUricAcidStandardvialitself. 3.AssayProbe |
Protocol 1.SamplePreparation Blood(serum/plasma),urine,otherbodyfluidscanbemeasureddirectlybyaseriesofdilutionsofthesample(1/2;1/4;1/8;…)toensurethereADIngsarewithinthestandardcurverange.YoursamplescanbedilutedwithdH2OordilutedUricAcidAssayBuffer. Note: |
2.StandardCurvePrepation Add50μl,40μl,30μl,20μl,10μl,5μl,1μl,and0μlofdilutedUricAcidStandardtoeachwell,thenadjustvolumeto50μl/wellwithdilutedUricAcidAssayBuffer. |
3.ReactionSolutionPreparation |
4.Performtheassay a.Mixthereactionsolutionwellandadd50μlofthereactionsolutiontoeachwellcontainingtheUricAcidstandardsandtestsamples. |
5.Calculation a.AveragetheOD570nmvaluesofreplicatewellsofeachUricAcidStandard,testsamples,andblank.Then,correctbackgroundbysubtractingthevaluederivedfromtheblankfromallsamplereadings. UricAcid(μM)=[(Correctedabsorbance)-(y-intercept)]/Slope |
MaterialSafetyDataSheet
Date:Oct26,2015
1.PRODUCTANDCOMPANYIDENTIFICATION
1.1Productidentifier
Productname:UricAcidAssayKit
CatalogNo:1200061002,120006100P
1.2Relevantidentifiedusesofthesubstanceormixtureandusesadvisedagainst
Forresearchuseonly.
1.3Detailsofthesupplierofthesafetydatasheet
Company:EtonBioscienceInc
TollFree:1-800-758-1630
Tel:1-800-758-1630
Fax:1-800-507-2912
1.4Emergencytelephonenumber
1-800-758-1630
2.HAZARDSIDENTIFICATION
2.1Classificationofthesubstanceormixture
Notahazardoussubstanceormixture
2.2GHSLabelelements,includingprecautionarystatements
Notahazardoussubstanceormixture
2.3Otherhazards
None
3.COMPOSITION/INFORMATIONONINGREDIENTS
3.1Substances
UricAcidStandard | UricAcidAssayEnzymeMix | |||||||
Components | CAS# | Components | CAS# | |||||
UricAcid | 69-93-2 | Uricase | 9002-12-4 | |||||
LithiumCarbonate | 554-13-2 | Tris-HClpH7.5 | N/A | |||||
Water | 7732-18-5 | Water | 7732-18-5 | |||||
UricAcidAssayBuffer | AssayProbe | |||||||
Components | CAS# | Components | CAS# | |||||
Tris-HClpH7.5 | N/A | DMSO | 67-68-5 | |||||
Water | 7732-18-5 | |||||||
EyeProtection | Wearsafetygoggles |
SkinProtection | Wearprotectiveclothing |
HandProtection | Wearprotectivegloves |
Respiratoryprotection | WearNIOSH/MSHAapprovedrespirators |
9.PHYSICALANDCHEMICALPROPERTIES
9.1Informationonbasicphysicalandchemicalproperties
UricAcidAssayBuffer
Appearance | Liquid,colorless |
pH | 7.5 |
Meltingpoint | Nodataavailable |
Freezingpoint | Nodataavailable |
Boilingpoint | Nodataavailable |
Density | Nodataavailable |
Watersolubility | Soluble |
UricAcidStandard
Appearance | Liquid,colorless |
pH | Nodataavailable |
Meltingpoint | Nodataavailable |
Freezingpoint | Nodataavailable |
Boilingpoint | Nodataavailable |
Density | Nodataavailable |
Watersolubility | Soluble |
UricAcidAssayEnzymeMix
Appearance | Liquid,colorless |
pH | Nodataavailable |
Meltingpoint | Nodataavailable |
Freezingpoint | Nodataavailable |
Boilingpoint | Nodataavailable |
Density | Nodataavailable |
Watersolubility | Soluble |
AssayProbe
Appearance | Liquid,colorless |
pH | Nodataavailable |
Meltingpoint | Nodataavailable |
Freezingpoint | Nodataavailable |
Boilingpoint | Nodataavailable |
Density | Nodataavailable |
Watersolubility | Soluble |
9.2Othersafetyinformation
Nodataavailable.
10.STABILITYANDREACTIVITY
10.1Reactivity
Nodataavailable
10.2Chemicalstability
Stableunderrecommendedstorageconditions.
10.3Possibilityofhazardousreactions
Nodataavailable.
10.4Conditionstoavoid
Nodataavailable
10.5IncompatIBLematerials
Strongoxidizingagents
10.6Hazardousdecompositionproducts
Hazardousdecompositionproductsformedunderfireconditions:carbonoxides
11.TOXICOLOGICALINFORMATION
11.1Informationontoxicologicaleffects
Toxicologicaleffectsonthisproducthavenotbeenthoroughlystudied.
12.ECOLOGICALINFORMATION
12.1Toxicity
Avoidreleaseintoenvironment
12.2Persistenceanddegradability
Nodataavailable
12.3.Bioaccumulativepotential
Nodataavailable
12.4Mobilityinsoil
Nodataavailable
12.5ResultsofPBTandvPvBassessment
Nodataavailable
12.6Otheradverseeffects
Nodataavailable
13.DISPOSALINFORMATION
13.1Wastetreatmentmethods
Disposeinaccordancewithlocal,state,andfederalregulations.
14.TRANSPORTINFORMATION
DOT:
Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport
IATA:
Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport
ADR/RID
Propershippingname:none
Non-Hazardousfortransport:thissubstanceisconsideredtobenon-hazardousfortransport
15.REGULATORYINFORMATION
EURiskandSafetyphrases:
R36/37/38:irritatingtoeyes/respiratorysystem/skin
16.OTHERINFORMATION
Theinformationaboveisbelievedtobeaccurateandrepresentsthebestinformationcurrentlyavailabletous.However,wemakenowarrantyofmerchantabilityoranyotherwarranty,expressorimplied,withrespecttosuchinformation.EtonBioscienceInc.shallnotbeheldliableforanydamagesorotherconsequencesresultingfromhandlingorfromcontactwiththeaboveproduct.Esquivel,CarlosJ.,BryanJ.Cassone,andPeterM.Piermarini.“AdeNovoTranscriptomeoftheMalpighianTubulesinNon-Blood-FedandBlood-FedAsianTigerMosquitoesAedesAlbopictus:InsightsintoDiuresis,Detoxification,andBloodMealProcessing.”Ed.StephenTobe.PeerJ4(2016):e1784.PMC.Web.9Aug.2017.
