Description: MCL, Rat, mAb WEN42

The monoclonal antibody WEN42 recognizes rat macrophage C-type lectin (MCL). C-type lectin receptors (CLRs) have recently been identified as a fourth family of pattern-recognition receptors for pathogen associated molecular patterns, following TLRs, Nod-like receptors and RIG-I-like receptors. MCL is a membrane surface receptor encoded by the Antigen Presenting Lectin-like gene Complex (APLEC).The complex consists of seven related C-type lectin receptor genes, namely, Dendritic Cell Activating Receptor (DCAR), Dendritic Cell Inhibitory Receptor 1, 2, 3 and 24 (DCIR), Macrophage C-type lectin (MCL) and Macrophage inducible C-type lectin (Mincle). MCL is a type II transmembrane protein with a single extracellular C-terminal C-type lectin-like domain. C-type lectins are functionally diverse. Human MCL is expressed on macrophages (synovial, peritoneal, and blood monocyte-derived), Langerhans cells and on neutrophils, monocytes and immature and mature DCs. In rats, expression of MCL has been detected on macrophages, neutrophils, DCs, B cells, and T cells. Their presence on the surface of immune cells and their potential for recognizing polysaccharide structures suggests a central role as pattern-recognition receptors (PRR) in the innate immune system. Despite the growing amount of data describing expression and function of the APLEC receptors, very little has been reported about MCL in general and the rat MCL in particular. Studies investigating the role of MCL has demonstrated that MCL is capable of inducing phagocytosis, cytokine production and oxidative burst, suggesting an activating role for this protein in the immune response. WEN42 does not bind to cells transfected with human MCL or with other APLEC receptors.

Specifications:

Catalog number Product type Quantity Formulation Application Application Notes Use Immunogen Isotype Species Alias Positive Control References Storage and stability Precautions Disease

HM3042

Monoclonal antibodies

100 µg

1 ml (100 µg/ml) 0.2 µm filtered antibody solution in PBS, containing 0.1% bovine serum albumin.

Flow cytometry, Frozen sections, Functional studies, Immuno precipitation, Western blot

F: Frozen splenic sections were fixed in acetone and incubated with WEN42. MHC class I was used as an isotype control (Ref.1). FC: Rat MCL is expressed on the extracellular domain of monocytes, macrophages and granulocytes (Ref.1). FS: beads coated with anti-MCL antibody increased phagocytosis in the RMW cells (Ref. 1).

For immunohistochemistry, flow cytometry, immunoprecipitation and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

BWZ.36 cell expressing chimeric MCL

Mouse IgG2a

Rat

Marcophage C-type lectin, CLR, CLECS89, CLEC4D

Cells of myeloid origin, MCL-transfected 293 cells or CHO cells.

1. Lobato-Pascual, A et al; Rat macrophage C-type lectin is an activating receptor expressed by phagocytic cells. PLoS ONE 2013, 8: e57406 2. Lobato-Pascual, A et al; Mincle, the receptor for mycobacterial cord factor, forms a functional receptor complex with MCL and FcεRI-γ. Eur J Immunol 2013, 43: 3167

Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product.

Infectious diseases

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