Description: IP-10, Human, mAb 6D4, biotinylated
The monoclonal antibody 6D4 recognizes human C-X-C motif chemokine 10 (IP-10), a protein of 98 amino acids. IP-10, also known as CXCL10, functions as ligand for the CXCR3 receptor. IP-10 belongs to the α-chemokine (C-X-C) family, which can be divided in two subfamilies: (1) potent chemoattractants for neutrophils, like IL-8 and (2) potent chemoattractants for lymphocytes, like the IFN inducible protein (IP)-10. IP-10 is produced by a wide variety of cell types ranging from neutrophils, dendritic cells and monocytes to hepatocytes, endothelial cells and keratinocytes. The cytokine is reported to be involved in a scala of inflammatory pathologies such as HIV, encephalitis, cutaneous T cell lymphoma, chronic hepatitis, psoriasis and acute anterior uveitis. Various observations strongly suggest a role for the C-X-C chemokines IL-8 and IP-10 in the regulation of angiogenic activity in cancer and in idiopathic pulmonary fibrosis. Furthermore IP-10 is associated with acute rejection processes estimated by the predictive properties of urinary IP-10 expression for the short- and long-term graft function after kidney transplantation.Specifications:
| HM2031 |
| Monoclonal antibodies |
| 50 µg |
| 0.5 ml (100 μg/ml) 0.2 μm filtered protein G purified biotinylated antibody solution in PBS, containing 0.1% bovine serum albumin and 0.02% sodium azide. |
| Biotin |
| Flow cytometry, Frozen sections, Immuno assays, Immuno fluorescence, Western blot |
| FC: Antibody 6D4 stains human CXCL10. For intracellular staining activated plasmacytoid dendritic cells were fixed with 2% paraformaldehyde and permeabilized with 0.5% saponin. All steps were performed in presence of 0.5 M EDTA to avoid cell aggregation (Ref.3)W: Tissue was homogenized in loading sample buffer and subjected to SDS-PAGE. The band size is ~9 kDa (Ref.4).IHC-F: Tissue sections were fixed 10 minutes in acetone and pretreated with avidin/biotin blocking kit. As positive control chronically inflamed liver was used and as negative control normal liver and isotype matched antibody (Ref.4). |
| Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. |
| Recombinant human IP-10 |
| Mouse IgG2a |
| Human |
| 10 kDa interferon gamma-induced protein, CXCL10, Small-inducible cytokine B10Gene name: CXCL10 |
| Acitvated plasmacytoid dendritic cells |
| Normal liver |
| 1. Hamamdzic, D. et al; Reovirus triggers cell type-specific proinflammatory responses dependent on the autocrine action of IFNβ. Am J Physiol Lung Cell Mol Physiol 2001, 280: L182. Giustizieri, M et al; Nitric oxide donors suppress chemokine production by keratinocytes in vitro and in vivo. Am J Path 2002, 161: 14093. Bendriss-Vermare, N et al; Virus overrides the propensity of human CD40L-activated plasmacytoid dendritic cells to produce Th4 mediators through synergistic induction of IFN-gamma and Th1 chemokine production. J of Leukoc Biol. 2005, 78: 9544. Curbishley, S et al; CXCR3 activation promotes lymphocyte transendothelial migration across human hepatic endothelium under fluid flow. Am J Pathol 2005, 167: 8875. Wetzel, M et al; μ-Opioid Induction of Monocyte Chemoattractant Protein-1, RANTES, and IFN-g-Inducible Protein-10 Expression in Human Peripheral Blood Mononuclear Cells. J Immunol 2000, 165: 6519 |
| Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label. |
| For research use only. Not for use in or on humans or animals or for diagnostics. It is the responsibility of the user to comply with all local/state and federal rules in the use of this product. Hycult Biotech is not responsible for any patent infringements that might result from the use or derivation of this product. |
| Infectious diseases |
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