快报
欢迎光临蚂蚁淘在线！ 请登录 免费注册
商品
品牌
文章
热门搜索：
快报
品牌首页 品牌新闻 品牌简介 产品中心 品牌咨询 联系我们
品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / Athens Research / Athens Research/Cambio - Excellence in Molecular Biology/0.5ml/EZT-RBL2-1-05

Athens Research/Cambio - Excellence in Molecular Biology/0.5ml/EZT-RBL2-1-05

价格
面议
货号：EZT-RBL2-1-05
浏览量：127
品牌：Athens Research
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Transfection - EZ Biosystems

Transfection - EZ Biosystems: Cell Lines

Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.

For primary cells click HERE

To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE

  • Catalogue
  • Description

RBL-2H3 Cell Avalanche™ Transfection Reagent (rat leukemia cell)

RBL-2H3 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting RBL-2H3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for RBL-2H3 Cells

EZ Biosystems

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
  • €
EZT-RBL2-1-05RBL-2H3 CellTransfection Reagent (rat leukemia cell)0.5ml£399.00QuantityAdd to Order
EZT-RBL2-1-15RBL-2H3 CellTransfection Reagent (rat leukemia cell)1.5ml£699.00QuantityAdd to Order

RBL-2H3 Cell Avalanche™ Transfection Reagent (rat leukemia cell)

RBL-2H3 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting RBL-2H3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for RBL-2H3 Cells

EZ Biosystems

Cell Line Information:

Designations: RBL-2H3 Depositors: RP Siraganian Growth Properties: adherent Organism: Rattus norvegicus deposited as Rattus sp. Morphology: fibroblast Source: peripheral blood Strain: Wistar Disease: basophilic leukemia Cell Type: basophil; chemically induced Cellular Products: histamine Applications: transfection host Receptors: FcERI (Fc of IgE) Comments: RBL-2H3 is a basophilic leukemia cell line isolated and cloned in 1978 from Wistar rat basophilic cells that were maintained as tumors. These cells have high affinity IgE receptors. They can be activated to secrete histamine and other mediators by aggregation of these receptors or with calcium ionophores. They have been used extensively to study FcERI and the biochemical pathways for secretion in mast cells. They have also been used extensively for studies of different aspects of secretion in cells including the role of changes in intracellular calcium, the activation of phospholipases, protein kinases and small G proteins.

The Transfection Reagent:

RBL-2H3 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting RBL-2H3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for RBL-2H3 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

  • Unique formulation-maximize transfection performance in RBL-2H3 Cells.
  • Extremely gentle to cells
  • Deliver single or multiple plasmids
  • Simplest and straightforward protocols
  • Compatible with serum
  • Compatible with transfection in any plate formats
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Developed and manufactured by EZ Biosystems
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on RBL-2H3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for RBL-2H3 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this RBL-2H3 Cell Avalanche Transfection Reagent.
For Other Cells:
RBL-2H3 Cell Avalanche™ Transfection Reagent (rat leukemia cell) can also be used on the following cells with high transfection efficiencies.

786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Myeloperoxidase Enzyme Immunoassay Kit 髓过氧化物酶 免疫分析试剂盒 Human MPO EIA KIT FEATURES: USE - Measure human MPO in a variety of matrices SAMPLE -Serum, Platelet-Poor Heparin Plasma, Saliva, Urine or Tissue Culture Media SAMPLES / KIT - 40 in duplicate SENSITIVITY - 0.068 ng/mL STABILITY - liquid reagents stable at 4°C QUICK RESULTS - 2.5 HOURS Myeloperoxidase (MPO) is a tetrameric heme-containing protein abundantly produced in neutrophil granulocytes where it plays an important anti-microbial role. During degranulation MPO is released into the extracellular space. There, as part of the neutrophils “respiratory burst”, it produces hypochlorous acid from hydrogen peroxide and Cl–. MPO also uses hydrogen peroxide to oxidize tyrosine to the tyrosyl radical. Both hypochlorous acid and tyrosyl are cytotoxic and when present can kill bacteria and other pathogens. Hereditary deficiency of myeloperoxidase predisposes individuals to immune deficiency. Studies have shown an association between elevated MPO levels and coronary artery disease, and in 2003 it was suggested that MPO may serve as a sensitive predictor of myocardial infarction in patients complaining of chest pain. Since that time the clinical utility of MPO testing in cardiac patients has been solidly established in the literature with well over 100 papers published. In 2010 this clinical application was further refined by additional studies which determined that measuring both MPO and C-reactive protein (CRP) provided more accurate prediction of mortality risk than measuring just CRP alone.