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| Description | The Biosensis Mature BDNF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of mature BDNF in less than 3 hours in cell culture supernatants, serum, plasma (citrate and EDTA), cell lysates, human milk and brain extracts only if used as directed, with a simplified protocol and no loss of sensitivity or specificity. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples. Note that accurate quantification of BDNF in human milk requires a secretory IgA (sIgA) blocker which can be purchased separately (BL-001-1250). For measurement of mature BDNF in CSF samples, please contact us at sales@biosensis.com. This ELISA kit has been tested in independent research laboratories and found to achieve highest reproducibility with intra- and inter-assay CVs as low as 1% and 5%, respectively (Polacchini et al., 2015).This ELISA kit consists of a pre-coated mouse monoclonal anti-mature BDNF capture antibody, a biotinylated anti-mature BDNF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a coloured reaction product which is directly proportional to the concentration of mature BDNF present in samples and protein standards. A BDNF positive control (QC sample) is provided to assure consistent assay performance.This Mature BDNF ELISA kit employs a recombinant human mature BDNF standard approved by the World Health Organization (WHO, www.nibsc.org). The amino acid sequence of mature BDNF is identical for human, mouse, rat and a number of other species. This kit therefore is suitable to measure mature BDNF in all these species and uses the same antibodies and antigen. This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures. For in-vitro diagnostic (IVD) applications in the European Economic Area (EEA), we refer to the CE Marked BDNF RapidTM ELISA kit (BEK-2211-CE). |
| Related products | BL-001-1250, Secretory IgA (sIgA) blocker: Sample diluent additive for accurate quantification or BDNF in human milk. BEK-2237-1P: Human proBDNF Rapid ELISA Kit BEK-2240: Mature BDNF/proBDNF Combo Rapid ELISA Kit |
| Batch No. | Refer to the product label. |
| Antigen | BDNF belongs to the neurotrophin family and regulates the survival and differentiation of neurons during development. The alterations in BDNF expression induced by various kinds of brain insult including stress, ischemia, seizure activity and hypoglycemia, may contribute to some pathologies such as depression, epilepsy, Alzheimers Disease, and Parkinsons disease. FUNCTION: BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. BDNF is also a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB. SUBCELLULAR LOCATION: Secreted protein. Post Translation Modification (PTM): The propeptide is N-glycosylated and glycosulfated. PTM: Converted into mature BDNF by plasmin (PLG) . DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. SIMILARITY: Belongs to the NGF-beta family. |
| Other Names | Brain-derived neurotrophic factor; BDNF; Abrineurin |
| Accession | P23560 BDNF_HUMAN; |
| Specificity | Human, mouse, rat and numerous other species. |
| Cross-reactivity | No cross-reactivity is observed for nerve growth factor (NGF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL in assay buffer. The reactivity of full-length proBDNF (0.125 ng/mL 5 ng/mL) was determined in six independent assays using proBDNF proteins from four different sources (mammalian and bacterial, wild-type and mutated). The average cross-reactivity of proBDNF was found to be 5.3% ± 0.5% in weight (w/v) concentration, or 12.1% ± 1.2% in molar concentration (mean ± SEM). |
| Storage | Store at 4°C |
| Expiry Date | 12 months from purchase. |
| Specific References | Reidling JC et al. (2017) Human Neural Stem Cell Transplantation Rescues Functional Deficits in R6/2 and Q140 Huntingtons Disease Mice. Stem Cell Reports. [In press] doi.org/10.1016/j.stemcr.2017.11.005. Application: Mouse brain homogenates.Perricos A, Ashjaei K, Husslein H, Proestling K, Kuessel L, Obwegeser R, Wenzl R, Yotova I (2017) Increased serum levels of mBDNF in women with minimal and mild endometriosis have no predictive power for the disease. Exp Biol Med (Maywood). [Epub ahead of print] doi: 10.1177/1535370217742600. Application: Human serum.Inoue T, Ninuma S, Hayashi M, Okuda A, Asaka T, Maejima H (2017) Effects of long-term exercise and low-level inhibition of GABAergic synapses on motor control and the expression of BDNF in the motor related cortex. Neurol Res. 11:1-8 doi: 10.1080/01616412.2017.1382801. Application: Mouse brain homogenates.Pedard M, Demougeot C, Prati C, Marie C (2017) Brain-derived neurotrophic factor in adjuvant-induced arthritis in rats. Relationship with inflammation and endothelial dysfunction. Prog Neuropsychopharmacol Biol Psychiatry. pii: S0278-5846(17)30730-3; [Epub ahead of print] doi: 10.1016/j.pnpbp.2017.11.006. Application: Rat serum.Wu YJ, Lin CC, Yeh CM, Chien ME, Tsao MC, Tseng P, Huang CW, Hsu KS (2017) Repeated transcranial direct current stimulation improves cognitive dysfunction and synaptic plasticity deficit in the prefrontal cortex of streptozotocin-induced diabetic rats. Brain Stimul. 2017 Aug 24; [Epub ahead of print] doi: 10.1016/j.brs.2017.08.007. Application: Rat brain homogenates.Yotova I, Hsu E, Do C, Gaba A, Sczabolcs M,Dekan S, Kenner L, Wenzl R, Tycko B (2017) Epigenetic Alterations Affecting Transcription Factors and Signaling Pathways in Stromal Cells of Endometriosis. PLoS One. 2017 Jan 26;12(1):e0170859. doi: 10.1371/journal.pone.0170859. Application: Human primary cell line supernatants.Guerzoni LPB, Nicolas V, Angelova A (2016) In Vitro Modulation of TrkB Receptor Signaling upon Sequential Delivery of Curcumin-DHA Loaded Carriers Towards Promoting Neuronal Survival. Pharm Res. [Epub ahead of print]. doi:10.1007/s11095-016-2080-4. Application: Human cell line supernatants and RIPA extracts.Riffault B, Kourdougli N, Dumon C, Ferrand N, Buhler E, Schaller F, Chambon C, Rivera C, Gaiarsa JL, Porcher C (2016) Pro-Brain-Derived Neurotrophic Factor (proBDNF)-Mediated p75NTR Activation Promotes Depolarizing Actions of GABA and Increases Susceptibility to Epileptic Seizures. Cereb. Cortex [Epub ahead of print]. doi: 10.1093/cercor/bhw385. Application: Rat cortex and hippocampus RIPA extracts.Khaing ZZ, Agrawal NK, Park JH, Xin S, Plumton GC, Lee KH, Huang YJ, Niemerski AL, Schmidt CE, Grau JW (2016) Localized and Sustained Release of Brain-Derived Neurotrophic Factor from Injectable Hydrogel/Microparticle Composites Fosters Spinal Learning after Spinal Cord Injury. J Mater Chem B. [Epub ahead of print]. doi: 10.1039/C6TB01602B. Application: In vitro release of BDNF from HAMC hydrogels.Turnbull MT and Coulson EJ (2016) Cholinergic Basal Forebrain Lesion Decreases Neurotrophin Signaling without Affecting Tau Hyperphosphorylation in Genetically Susceptible Mice. J Alzheimers Dis. [Epub ahead of print]. doi: 10.3233/JAD-160805. Application: Mouse hippocampus extracts.Brandli A, Johnstone DM, Stone J (2016) Remote Ischemic Preconditioning Protects Retinal Photoreceptors: Evidence From a Rat Model of Light-Induced Photoreceptor Degeneration. Invest Ophthalmol Vis Sci. 57(13):5302-13. doi: 10.1167/iovs.16-19361. Application: Rat serum and retinal RIPA extracts.Chrysostomou V, Galic S, van Wijngaarden P, Trounce IA, Steinberg GR, Crowston JG (2016) Exercise reverses age-related vulnerability of the retina to injury by preventing complement-mediated synapse elimination via a BDNF-dependent pathway. Ageing Cell. 2016 Sep 9. doi: 10.1111/acel.12512. [Epub ahead of print]. Application: Mouse serum, mouse retina and mouse muscle homogenates (Tris/Triton buffer).Uys M, Shahid M, Sallinen J, Dreyer W, Cockeran M, Harvey BH (2016) The α2C-adrenoceptor antagonist, ORM-10,921, has antipsychotic-like effects in social isolation reared rats and bolsters the effects of haloperidol. Prog Neuropsychopharmacol Biol Psychiatry. 2016 Jul 2. pii: S0278-5846(16)30105-1. doi: 10.1016/j.pnpbp.2016.07.002. [Epub ahead of print]. Application: Acid-extracted rat brain homogenate.Polacchini A, Metelli G, Francavilla R, Baj G, Florean M, Mascaretti LG, Tongiorgi E (2015) A method for reproducible measurements of serum BDNF: comparison of the performance of six commercial assays. Sci Rep. 10;5:17989. doi: 10.1038/srep17989. Application: Human serum.Hutton CP, Déry N, Rosa E, Lemon JA, Rollo CD, Boreham DR, Fahnestock M, deCatanzaro D, Wojtowicz JM, Becker S (2015) Synergistic effects of diet and exercise on hippocampal function in chronically stressed mice. Neuroscience 308:180-93. doi: 10.10166. Application: Mouse serum.Boskovic Z, Alfonsi F, Rumballe BA, Fonseka S, Windels F, Coulson EJ (2014) The role of p75NTR in cholinergic basal forebrain structure and function. J Neurosci. 34(39):13033-8. doi: 10.1523/JNEUROSCI.2364-14.2014. Application: Mouse brain homogenates. |
| Kit components | The ELISA kit box contains 1 x 96 pre-coated strip plate, protein standards, QC sample, detection reagents, wash and sample buffers and detailed protocols. |
| Range | 7.8 pg/mL - 500 pg/mL |
| Sensitivity | Typical limit of detection (LOD) for BDNF is less than 2 pg/mL, determined as 150% of the blank value. |
| Kit protocol | ![\"Click](\"https://www.ebiomall.com/images/pdf1.gif\" "\\"") BEK-2211_as_at_June2017.pdf |
| MSDS | MSDS_Rapid_ELISA_as_at_Aug2017.pdf |
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