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| Description | The human Insulin-like growth factor-binding protein 1 (IGFBP-1) Kit is a sandwich ELISA. The capture antibody is a polyclonal human IGFBP-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known IGFBP-1 concentration are added to these wells and allowed to complex with the bound IGFBP-1 antibody. A biotinylated human IGFBP-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of IGFBP-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human IGFBP-1 in samples such as sera, plasma, and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
| Batch No. | See product labels |
| Other Names | IGF-binding protein 1; IGFBP-1; IBP-1; IGFBP1; |
| Accession | P08833 IBP1_HUMAN; |
| Specificity | Human Insulin-like growth factor-binding protein 1 |
| Storage | Store at 4øC |
| Kit components | The ELISA kit box contains 1 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions. |
| Range | 31.2 pg/ml - 2,000 pg/ml |
| Sensitivity | < 1 pg/ml |
| Kit protocol | ![\"Click](\"https://www.ebiomall.com/images/pdf1.gif\" "\\"") BEK-2030-1P_as_at_Jan2017_protocol.pdf |
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