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| Description | The rat TNF-alpha Kit is a sandwich ELISA. The capture antibody is a monoclonal rat TNF-alpha antibody pre-coated onto the 96-well strip plates provided in the kit. Rat test samples and standards of known TNF-alpha concentration are added to these wells and allowed to complex with the bound TNF-alpha antibody. A biotinylated rat TNF-alpha polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TNF-alpha present in the samples. The purpose of this kit is the in-vitro quantitative determination of rat TNF-alpha in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
| Batch No. | See product labels |
| Antigen | Tumor necrosis factor alpha (TNF-alpha) is a cytokine that belongs to the tumor necrosis factor (TNF) superfamily. TNF-alpha is mainly secreted by macrophages. TNF-alpha is involved in the regulation of many processes such as� cell proliferation, differentiation and apoptosis and has been implicated in a variety of diseases including cancer. TNF-alpha is expressed as a single-pass type II membrane protein. A soluble, secreted form is derived from the membrane form by proteolytic processing. |
| Other Names | TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a; Cachectin; TNF; TNFA; TNFSF2; |
| Accession | P16599 TNFA_RAT; |
| Specificity | Rat TNF-alpha |
| Storage | Store at 4°C |
| Kit components | The ELISA kit box contains 1 x 96 pre-coated strip plate, protein standards, detection reagents, substrate buffer and precise instructions. |
| Range | 15.6 pg/ml - 1,000 pg/ml for body fluids, tissue lysates or cell culture supernates. 7.8 pg/ml - 500 pg/ml for rat sera. |
| Sensitivity | < 1 pg/ml |
| Kit protocol | ![\"Click](\"https://www.ebiomall.com/images/pdf1.gif\" "\\"") BEK-2101-1P_as_at_Jun2017.pdf |
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