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Description | The human Lipocalin-2 Kit is a sandwich ELISA. The capture antibody is a polyclonal human Lipocalin-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Lipocalin-2 concentration are added to these wells and allowed to complex with the bound Lipocalin-2 antibody. A biotinylated human Lipocalin-2 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Lipocalin-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Lipocalin-2 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
Batch No. | See product labels |
Antigen | The standard and antigen for this Lipocalin-2/NGAL ELISA is recombinant human Lipocalin-2/NGAL amino acids 21-N198 produced in an purified from a mammalian cell expression system. |
Other Names | Neutrophil gelatinase-associated lipocalin; NGAL; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; p25; LCN2; HNL; |
Accession | P80188 NGAL_HUMAN; |
Specificity | Human Lipocalin-2/NGAL |
Storage | Store at 4øC |
Kit components | The ELISA kit box contains 1 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions. |
Range | 156 pg/ml - 10,000 pg/ml |
Sensitivity | < 10 pg/ml |
Kit protocol | Please contact us at biospeak@biosensis.com for a detailed protocol for this ELISA kit. |
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