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| Description | The human Metalloproteinase inhibitor 1 (TIMP-1) Kit is a sandwich ELISA. The capture antibody is a polyclonal human TIMP-1 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known TIMP-1 concentration are added to these wells and allowed to complex with the bound TIMP-1 antibody. A biotinylated human TIMP-1 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of TIMP-1 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human TIMP-1 in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
| Batch No. | See product labels |
| Antigen | The matrix metalloproteinases (MMPs) are a large family of¶ÿ zinc endopeptidases that¶ÿ degrade extracellular matrix components such as fibronectin and collagen type III. The tissue inhibitors of metalloproteinase (TIMPs) complex with these matrix metalloproteinases and irreversibly inactivate them. The TIMP proteins are¶ÿ homologous and are either secreted in soluble form (TIMP1, TIMP2 and TIMP4) or bind to extracellular matrix components (TIMP3). |
| Other Names | Tissue inhibitor of metalloproteinases 1; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; EPA; |
| Accession | P01033 TIMP1_HUMAN; |
| Specificity | Human TIMP-1 |
| Storage | Store at 4øC |
| Kit components | The ELISA kit box contains 1 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions. |
| Range | 156 pg/ml - 10, 000 pg/ml |
| Sensitivity | < 5 pg/ml |
| Kit protocol | Please contact us at biospeak@biosensis.com for a detailed protocol for this ELISA kit. |
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