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苏州工业园区生物纳米园A4#216
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商品描述
| Description | The6XHistagisashortpeptidesequenceof6histidineresidues.Epitopessuchasthe6XHistagareoftenincludedwiththetargetDNAatthetimeofcloningtoproducefusionproteinscontainingthetagsequence.Thisallowsanti-epitopetagantibodiessuchasthisonetoserveasauniversaldetectionreagentforanyrecombinantproteincontainingthistag.Anti-epitopeantibodiesareausefulalternativetogeneratingantibodiestoidentifyaspecificrecombinantprotein.The6XHismotifisoftenusedasatagonrecombinantproteinstofacilitatepurificationwithimmobilizedmetal-affinitychromatography. |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Syntheticpeptidecontaining6Histidineresidues. |
| Isotype | IgG2b |
| Producedin | Mouse.MonoclonalclonenumberHIS.H8 |
| Applications | WesternBlotting(WB),Immunocytochemistry(IC)andImmunoprecipitation(IP).Suggestedstartingdilutionsareasfollows:WBatdilutionsof1:1000,ICandIPatdilutionsof1:200.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | His-taggedfusionproteins |
| Form | Liquid.PBS,pH7.4with0.05%sodiumazide. |
| Storage | Stablefor1yearat-20°Cunopened.Afteropeningwerecommendaliquotingandstoringat-20°Cforupto6months.Avoidrepeatedfreezingandthawing.Formaximumrecoveryofproduct,centrifugetheoriginalvialafterthawingandpriortoopeningthecap. |
| ExpiryDate | 12monthsafterdateofreceipt,unopened. |
| SpecificReferences | 1.DittmannK.etal(2010)"NuclearEGFRshuttlinginducedbyionizingrADIationisregulatedbyphosphorylationatresidueThr654"FEBSLett.2010Sep24;584(18):3878-3884. 2.KolevM.V.etal(2010)"UpregulatingCD59:anewstrategyforprotectionofneuronsfromcomplement-mediateddegenerationPharmacogenomicsJ.2010Feb;10(1):12-9. |
| References | 1.PotalaS.andVermaR.S.(2010)"Targetingheadandnecksquamouscellcarcinomausinganovelfusiontoxin-diphtheriatoxin/HN-1."MolBiolRep.2010Aug1. |
