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| Description | ThemouseCystatinCKitisasandwichELISA.ThecaptureantibodyisapolyclonalmouseCystatinCantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.TestsamplesandstandardsofknownCystatinCconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundCystatinCantibody.Abiotinylatedmousepolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofCystatinCpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseCystatinCinsamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. |
| BatchNo. | Seeproductlabels |
| OtherNames | Cystatin-C;Cystatin-3;CST3; |
| Accession | P21460CYTC_MOUSE; |
| Specificity | CystatinC |
| Storage | Storeat4°C |
| Kitcomponents | TheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions. |
| Range | .312ng/ml-20ng/ml |
| Sensitivity | < 10 pg/ml |
| Kitprotocol |  BEK-2190-2P_as_at_May2015_protocol.pdf |