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Biosensis/Human Interleukin-6 (IL-6)/Interferon beta-2 (IFN-beta-2/B-cell stimulatory factor 2 (BSF-2) ELISA Kit (1 plate)/BE

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¥8340.00
货号:BEK-2042-1P
浏览量:118
品牌:Biosensis
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DescriptionThehumanInterleukin6(IL-6)kitisasandwichELISA.ThecaptureantibodyisamonoclonalhumanIL-6antibodypre-coatedontothe96-wellstripplatesprovidedinthekit.HumantestsamplesandstandardsofknownIL-6concentrationareaddedtothesewellsandallowedtocomplexwiththeboundIL-6antibody.AbiotinylatedhumanIL-6polyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofIL-6presentinthesamples.ThepurposeofthiskitisforquantitativedetectionofhumanIL-6incellculturesupernatants,serum,andheparin,EDTAorcitratetreatedplasmasamplesonlywhenusedasdirected.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.Seeproductlabels
AntigenThestandardproductusedinthiskitisrecombinanthumanIL-6,consistingaminoacids29-212expressedinandpurifiedfromanE.coliexpressionsystem
OtherNamesIL-6;Interleukin-6;B-cellstimulatoryfactor2;BSF-2;Interferonbeta-2;Hybridomagrowthfactor;CTLdiffenrentiationfactor;CDF;IL6;IFNB2;
AccessionP05231IL6_HUMAN;
SpecificityHumanIL-6
StorageStoreat4øC
SpecificReferencesWickströmK,Stavréus-EversA,VercauterenO,OlovssonM,EdelstamG(2016)"EffectofLignocaineonIL-6,IL-8,andMCP-1inPeritonealMacrophagesandEndometrioticStromalCells"ReproductiveSciences;[Epubaheadofprint].Application:Cellculturesupernatant.
KitcomponentsTheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range4.7pg/ml-300pg/ml
Sensitivity< 0.3 pg/ml
KitprotocolClick to download the kit protocolBEK-2042-1P_as_at_Nov2017_protocol.pdf