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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Human TrkB-Fc Chimera expressed in mammalian cells/PE-1235-20/20 µg
商品描述
| Description | TyrosineKinaseReceptorB(TrkB)isahighaffinityreceptorforbrain-derivedneurotrophicfactor(BDNF)andneurotrophin-4/5.Italsobindsneurotrophin3(NT-3)withloweraffinity.TrkBdoesnotbindnervegrowthfactor(NGF).TrkBisinvolvedinthedevelopmentand/ormaintenanceofthenervoussystemandiswidelyexpressed,predominantlyinnervoustissue.TrkBisaheavilyglycosylatedtypeItransmembraneprotein.TheTrkB-Fcchimeracontains13potentialN-linkedglycosylationsites. |
| BatchNo. | Seeproductlabel |
| Unitsize | 20µg |
| OtherNames | Tropomyosin-relatedkinasereceptor;BDNF/NT-3growthfactorsreceptor;Neurotrophictyrosinekinasereceptortype2;TrkBtyrosinekinase;GP145-TrkB/GP95-TrkB;Trk-B;Ntrk2;Trkb;TrkB-FcChimera |
| Accession | Q16620NTRK2_HUMAN; |
| Producedin | Human-ADNAsequenceencodingthesignalpeptideandextracellulardomainofhumanTrkB(aminoacids1-428)wasfusedtotheFcregionofhumanIgG1(aminoacids93-330).Thechimericproteinwasexpressedinmodifiedhuman293cells. |
| MolecularWeight | TheTrkB-Fcchimeramigratesasabroadbandbetween90and115kDainSDS-PAGEduetopost-translationalmodifications,inparticularglycosylation.TheunmodifiedTrkB-Fcchimerahasapredictedmolecularmassof71.0kDa.ThemodifiedTrkB-FcchimeraseparatesintoanumberofisoformswithapIbetween4.9and6.7in2DPAGEwhereastheunmodifiedTrkB-FcchimerahasapredictedpIof5.79. |
| Purity | >95%,asdeterminedbySDS-PAGEandvisualizedbysilverstain. |
| Applications | TheTrkB-FcchimeraboundtoproteinAsepharosebeadsisabletopulldownitsligandBDNF.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Form | Thechimeraconsistsof20-40%carbohydratebyweight. |
| Reconstitution | Itisrecommendedthat0.5mlofsterilephosphate-bufferedsalinebeaddedtothevial. |
| Storage | Lyophilizedproductsshouldbestoredat2to8°C.Followingreconstitution,short-termstorageat4°Cisrecommendedandlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended. |
