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蚂蚁淘在线 / 品牌中心 / Biosensis / Biosensis/Human Nerve Growth Factor Receptor-Fc Chimera (222 amino acids) expressed in mammalian cells/PE-1238-25/25 µg
商品描述
| Description | Nervegrowthfactor(NGF)receptor,alsoknownasp75NTR, isalowaffinityNGFreceptor.ItbindswithequalaffinityneurotrophinssuchasbetaNGF,BDNF,NT-3andNT-4.NGFreceptorsmediatesignalingofneurotrophinsforneuronalsurvival,apoptosis,neuriteoutgrowthandsynapticplasticity.ThesereceptorsarealsothoughttoplayaroleinneurogenerativediseasessuchasAlzheimersdisease.TheNGFreceptorisatypeItransmembraneglycoprotein(399aa)consistingofasignalpeptide(28aa),anextracellulardomain(222aa)whichcontainsfourcysteinerichdomainsresponsIBLeforligandbinding,atransmembranedomain(22aa)andacytoplasmicdomain(155aa). |
| BatchNo. | Seeproductlabel |
| Unitsize | 25µg |
| OtherNames | Low-affinitynervegrowthfactorreceptor;NGFreceptor;Gp80-LNGFR;p75ICD;Lowaffinityneurotrophinreceptorp75NTR;p75NTR;CD271; |
| Accession | P08138TNR16_HUMAN; |
| Producedin | Human-ADNAsequenceencodingthesignalpeptideandextracellulardomainofhumanNGFreceptor(aminoacids1-250)wasfusedtotheFcregionofhumanIgG1(aminoacids93-330).Thechimericproteinwasexpressedinmodifiedhuman293cells. |
| MolecularWeight | TheNGFReceptor-Fcchimeramigratesasabroadbandbetween75and100kDainSDS-PAGEduetopost-translationmodifications,inparticularglycosylation.TheunmodifiedNGFReceptor-Fcchimerahasapredictedmassofof50.8kDa.TheNGFReceptor-FcchimeraseparatesintoanumberofisoformswithapIbetween4.0and6.0in2DPAGE.TheunmodifiedNGFReceptor-FcchimerahasapredictedpIof4.89. |
| Purity | >95%,asdeterminedbySDS-PAGEandvisualizedbysilverstain |
| Biol.activity | TheED50oftheNGFReceptor-Fcchimeraistypically0.7-1.0ug/mlasmeasuredbyitsABIlitytoneutralizebetaNGFmediatedproliferationofthehumangrowth-factordependentTF-1cellline. |
| Form | TheNGFReceptor-Fcchimeraconsistsof30-50%carbohydratebyweight.Whenreconstitutedin0.5mlsterilephosphate-bufferedsaline,thesolutionwillcontain1%humanserumalbumin(HSA)and10%trehalose. |
| Reconstitution | Itisrecommendedthat0.5mlofsterilephosphate-bufferedsalinebeaddedtothevial. |
| Storage | Lyophilizedproductsshouldbestoredat2to8°C.Followingreconstitutionshort-termstorageat4°Cisrecommended,andlonger-termstorageofaliquotsat-18to-20°C.Repeatedfreezethawingisnotrecommended. |
